QCM Haptotaxis Cell Migration Assay -Fibronectin, 24-well, colorimetric
Recommended Replacement for: ECM500Description:
QCM Haptotaxis Cell Migration Assay -Fibronectin, 24-well, colorimetric
Trade Name:
- QCM
- Chemicon (Millipore)
Qty/Pk:
1 kit
Product Overview:
The CHEMICON QCM™ Haptotaxis Cell Migration Assay - Fibronectin, Colorimetric is ideal for the study of epithelial and fibroblast cell haptotaxis. The quantitative nature of this assay is especially useful for large scale screening of pharmacologic agents. BSA-coated control chambers provide an appropriate migration control. The 8 μm pore size of this assay's Boyden chambers is not appropriate for the study of lymphocyte migration.
• Each kit provides sufficient materials for the evaluation of 12 samples.
• Please refer to the product insert for assay details.
The CHEMICON QCM™ Haptotaxis Cell Migration Assay - Fibronectin, Colorimetric assay is intended for research use only; not for diagnostic applications.
• Each kit provides sufficient materials for the evaluation of 12 samples.
• Please refer to the product insert for assay details.
The CHEMICON QCM™ Haptotaxis Cell Migration Assay - Fibronectin, Colorimetric assay is intended for research use only; not for diagnostic applications.
Species Reactivity:
Vertebrates
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Components:
- Fibronectin Test Plate: (Part No. 2005689) One 24-well culture plate, containing 12 human FN-coated Boyden chambers, sufficient for the evaluation of 12 test samples.
- BSA Control Plate: (Part No. 2005791) One 24-well culture plate, containing 12 BSA-coated Boyden chambers, sufficient for the evaluation of 12 controls.
- Cell Stain Solution*: (Part No. 20294) One vial - 10 mL.
- Extraction Buffer: (Part No. 20295) One vial - 10 mL.
- 24 well Stain Extraction Plate: (Part No. 2005871). One plate.
- 96 well Stain Quantitation Plate: (Part No. 2005870). One plate.
- Swabs: (Part No. 10202) 50 each.
- Forceps: (Part No. 10203) 1 pair
- *Cell Stain Solution contains a small amount of crystal violet, which is toxic if swallowed or inhaled, and may cause irritation to the eyes, respiratory system, and skin. Handle with caution.
Brand Family:
Chemicon
Storage Conditions:
Store at 4°C for up to one year after date of purchase.
Detection Methods:
Chromogenic
Packaging:
Sufficient for analysis of 12 samples
Replaces:
ECM500
Product Name:
QCM Haptotaxis Cell Migration Assay -Fibronectin, 24-well, colorimetric
Materials Required but Not Delivered:
1. Precision pipettes: sufficient for aliquoting cells.
2. Harvesting buffer: EDTA or trypsin based cell detachment buffer, or other cell detachment formulations as optimized by individual investigators. Chemicon's ready-to-use non-mammalian detachment solution, Accutase™ (Cat. No. SCR005) can also be used.
3. Tissue culture growth medium appropriate for subject cells, such as DMEM containing 5% FBS.
4. Quenching Medium: serum-free medium, such as DMEM, MEM etc containing 5% BSA. Must contain divalent cations (Mg2+, Ca2+) sufficient for quenching EDTA in harvesting buffer.
5. Sterile PBS or HBSS to wash cells.
6. Distilled water
7. (Optional) Chemoattractant or pharmacological agent added to culture medium.
8. Low speed centrifuge and tubes for cell harvesting.
9. CO2 incubator appropriate for subject cells.
10. Hemocytometer or other means of counting cells.
11. Trypan blue or equivalent viability stain.
12. Microplate reader (540-570 nm detection) or spectrophotometer.
13. Sterile cell culture hood
14. (Optional) Graduated ocular (calibrated), or automated method for counting stained cells on a membrane.
15. Shaker
2. Harvesting buffer: EDTA or trypsin based cell detachment buffer, or other cell detachment formulations as optimized by individual investigators. Chemicon's ready-to-use non-mammalian detachment solution, Accutase™ (Cat. No. SCR005) can also be used.
3. Tissue culture growth medium appropriate for subject cells, such as DMEM containing 5% FBS.
4. Quenching Medium: serum-free medium, such as DMEM, MEM etc containing 5% BSA. Must contain divalent cations (Mg2+, Ca2+) sufficient for quenching EDTA in harvesting buffer.
5. Sterile PBS or HBSS to wash cells.
6. Distilled water
7. (Optional) Chemoattractant or pharmacological agent added to culture medium.
8. Low speed centrifuge and tubes for cell harvesting.
9. CO2 incubator appropriate for subject cells.
10. Hemocytometer or other means of counting cells.
11. Trypan blue or equivalent viability stain.
12. Microplate reader (540-570 nm detection) or spectrophotometer.
13. Sterile cell culture hood
14. (Optional) Graduated ocular (calibrated), or automated method for counting stained cells on a membrane.
15. Shaker