BCIP/NBT Single Reagent, Blue/purple, Alkaline Phosphatase Substrate (slightly soluble in alcohol)
Description:
BCIP/NBT Single Reagent, Blue/purple, Alkaline Phosphatase Substrate (slightly soluble in alcohol)
Trade Name:
Chemicon (Millipore)
Qty/Pk:
500 mL
Product Overview:
The most sensitive alkaline phosphatase substrate available for blotting techniques and in situ hybridization chemistry is the 5-bromo-4-chloro-3-indolyl phosphate/p-nitro blue tetrazolium system (BCIP/NBT). Using proprietary production and stabilization procedures, CHEMICON has made available a unique BCIP/NBT reagent that is stable at room temperature and several times more sensitive than existing formulations. For localization of enzyme labeled probes following Western, Northern, Southern or dot blotting, the reagent is added directly to the membrane. Following a 10-15 minute incubation, the reaction is terminated by distilled water rinses. Longer incubations may be employed if necessary, but use of biotin/streptavidin-alkaline phosphatase signaling complexes will negate prolonged reaction times.
Key Applications:
- Western Blotting
- Immunohistochemistry
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Color Code:
Black
Brand Family:
Chemicon
Presentation:
5-bromo-4-chloro-3-indolyl phosphate p-nitro blue tetrazolium chloride in a proprietary buffer and enhancer.
Storage Conditions:
Maintain at room temperature or 2-8°C for up to one year.
Reagent Type:
- ELISA Reagents
- Enzyme Substrates
Protein or Enzyme Type:
Enzyme Substrates
Product Name:
BCIP/NBT Single Reagent, Blue/purple, Alkaline Phosphatase Substrate (slightly soluble in alcohol)