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注文に関する情報
- : ESG1106
- : 106 units
- : 1 million units/1 mL
画像情報
Undifferentiated ES cells cultured for five days in media containing CHEMICON's ESGRO® (ESG1106 & ESG1107). A concentration of 1000 Units/mL is used f...
ES (R1) cell colonies (x10) cultured on gelatinized plates using ESGRO® (1000 units/mL).
ESGRO® (LIF)
Description:
ESGRO® (LIF)
Trade Name:
Chemicon (Millipore)
Qty/Pk:
106 units
Key Applications:
Stem Cell Culture
Application Notes:
Applications for ESGRO® include use as a reagent for the in vitro maintenance of the pluripotential phenotype of murine ES cells.
SUGGESTED PROTOCOLS:
ES Cells: In D3 and MBL-1 pluripotential ES cell cultures it is routinely found that 1000 U of ESGRO® per 1.0 mL of tissue culture media is required to maintain ES cells with a stem cell phenotype. Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells(E3).
At the recommended concentration 107 units of ESGRO® is sufficient for 10.0 L of tissue culture media and 106 units of ESGRO® is sufficient for 1.0 L of tissue culture media.
Visit www.esgro-lif.com for additional information
SUGGESTED PROTOCOLS:
ES Cells: In D3 and MBL-1 pluripotential ES cell cultures it is routinely found that 1000 U of ESGRO® per 1.0 mL of tissue culture media is required to maintain ES cells with a stem cell phenotype. Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells(E3).
At the recommended concentration 107 units of ESGRO® is sufficient for 10.0 L of tissue culture media and 106 units of ESGRO® is sufficient for 1.0 L of tissue culture media.
Visit www.esgro-lif.com for additional information
Species Reactivity:
Mouse
Usage Statement:
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
-
LABEL LICENSE - USE RESTRICTED TO RESEARCH USE ONLY
Leukemia Inhibitory Factor ("LIF") is protected by US Patents No. 5,443,825 and 5,750,654 and Canada Patent No. 1,341,581 and 1,341,469 owned by AMRAD Corporation Ltd. and licensed exclusively to Millipore for research uses. The use of this product is limited for research uses only and not for any commercial purposes, such as making or developing other research products from this product or use of this product as a component in a research kit. This product is not to be used in humans or for any diagnostic purposes.
The purchaser may refuse to accept the conditions of this label license by returning this product unopened and will receive a full refund of the purchase price paid.
For-profit entities or commercial research entities purchasing this product shall be required to execute a separate commercial license with Millipore within three months of purchase. Researchers may not transfer this product or its derivatives to researchers performing commercial research at other Nonprofit Organizations, or to For-Profit Organizations, without the express written consent of Millipore, and without those entities having an express license from Millipore for the use of the product. Other than as specifically provided herein, a transferee of this product shall have no right to transfer this product, or its derivatives to any other person or entity.
Entrez Gene Summary:
Leukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes.
UniProt Summary:
FUNCTION: SwissProt: P15018 # LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
SIZE: 202 amino acids; 22008 Da
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P15018 ## Belongs to the LIF/OSM family.
SIZE: 202 amino acids; 22008 Da
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P15018 ## Belongs to the LIF/OSM family.
Species:
Mouse
Cell Culture Media or Reagent Type:
ESGRO Supplement
Stem Cell Type:
- Mouse Embryonic Stem Cells
- Induced Pluripotent Stem Cells
Stem Cell Workflow Stage:
Maintenance & Expansion
Media Type:
Cell Culture Media Additives
Brand Family:
Chemicon
Format:
Purified
Presentation:
ESGRO® is supplied in liquid form as 106 Units ESGRO® in 1.0 mL of phosphate buffered saline with 1% w/v bovine serum albumin BSA as a carrier for stability.
UniProt Number:
Storage Conditions:
ESGRO® is shipped at ambient temperature. A cold pack may be included for customer relations, however it is truly not needed. Extensive stability tests have been performed with ESGRO® during development. This product is stable for at least 18 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells. For long term storage it is recommended that ESGRO concentrate be stored at 4°C. Freeze-thawing will reduce potency.It is recommended that prior to use, ESGRO™ should be diluted in sterile tissue culture media and aliquoted to a convenient concentration, then stored at 4°C. Freeze-thawing should be avoided.ESGRO™ is stable for a minimum of 7 days at 37°C, 5% CO2 incubator during the culture of ES cells.
WARRANTY
The highest standards of quality control are used in the manufacture of this product. No warranty is provided that the sale or use of the product either alone, in combination with other products, or in the operation of any process, will not infringe patent, intellectual property or any other rights of third parties. This product is supplied for research purposes only. It is not for human therapeutic and/or diagnostic use.NOTE: ESGRO® should not be allowed to come in contact with ruminant animals or swine.
Manufactured by CHEMICON International, Inc. LIF is protected under US Patent nos. 5,443,825, 5,750,654 and 6,261,548, European Patent no. 0285 448 and related foreign patents and is not available for resale. Use of recombinant LIF, or CHEMICON's proprietary ESGRO® formulation, for the maintenance of embryonic stem cells is protected world-wide under the following patents: US 5,166,065, EP380646, and others.
WARRANTY
The highest standards of quality control are used in the manufacture of this product. No warranty is provided that the sale or use of the product either alone, in combination with other products, or in the operation of any process, will not infringe patent, intellectual property or any other rights of third parties. This product is supplied for research purposes only. It is not for human therapeutic and/or diagnostic use.NOTE: ESGRO® should not be allowed to come in contact with ruminant animals or swine.
Manufactured by CHEMICON International, Inc. LIF is protected under US Patent nos. 5,443,825, 5,750,654 and 6,261,548, European Patent no. 0285 448 and related foreign patents and is not available for resale. Use of recombinant LIF, or CHEMICON's proprietary ESGRO® formulation, for the maintenance of embryonic stem cells is protected world-wide under the following patents: US 5,166,065, EP380646, and others.
Specific Activity:
ESGRO® is assessed both on ES {embryonic stem} cells (D3 & MBL-1) and on murine M1 myeloid leukemic cells(4). A standard of 50 Units is defined as the concentration of ESGRO® in 1.0mL of tissue culture medium that induces the differentiation of 50% of M1 colonies(4). 106 units is equivalent to approximately 10 μg of pure protein, and is sufficient to treat 1L of ES cell culture media.
Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay: Specific Activity >/= 108 units/mg
Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay: Specific Activity >/= 108 units/mg
Promotional Text:
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Packaging:
1 mL
Cell Culture Reagent Type:
- Cytokines & Growth Factors
- Cell Culture Supplements
Gene Symbol:
- LIF
- Emfilermin
- CDF
- MLPLI
- HILDA
- D-FACTOR
Protein or Enzyme Type:
Cytokines & Growth Factors
Product Name:
ESGRO® (LIF)
Entrez Gene Number:
Alternate Names:
Murine LIF
Concentration:
1 million units/1 mL
Purity:
The active component mLIF has been shown to be >95% pure by SDS-PAGE. ESGRO® supplied 0.22 micron sterile filtered, and tested negative for mycoplasmas.