Fibronectin 120 kDa α Chymotryptic Fragment (Cell Attachment region), Human purified

Fibronectin 120 kDa α Chymotryptic Fragment (Cell Attachment region), Human purified

Chemicon (Millipore)

500 µg

PROPERTIES:

Contains the cell attachment region of the fibronectin molecule.

Cell Culture

F1904 is suitable for use in ELISA and affinity chromatography, and possibly molecular mapping of fibronectin function.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.

FUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity).
SIZE: 2386 amino acids; 262607 Da
SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1.
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
PTM: Sulfated.
SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.

Human

• Human Embryonic Stem Cells
• Mesenchymal Stem Cells
• Neural Stem Cells
• Hematopoietic Stem Cells
• Epithelial Cells
• Pancreatic Stem Cells
• Induced Pluripotent Stem Cells

• Maintenance & Expansion
• General Cell Culture
• Differentiation

Chemicon

Human plasma cryoprecipitate (plasma fibronectin), assayed negative for hepatitis B virus, hepatitis C Virus (HCV), HIV-1, HIV-2, HTLV-1, HTLV-2 and Treponema pallidum. Handle as if potentially infectious.

Purified protein. Lyophilized in sodium citrate buffer, pH 7.4. Reconstitute with water.

P02751

Maintain material at -20°C for 3 months or -70°C for six months.

Attachment Factors

• FN1
• DKFZp686O13149
• DKFZp686H0342
• DKFZp686I1370
• MSF
• FN
• CIG
• DKFZp686F10164
• LETS
• FINC

Extracellular Matrix Proteins

Fibronectin 120 kDa α Chymotryptic Fragment (Cell Attachment region), Human purified

• NM_002026.2
• NM_054034.2
• NM_212474.1
• NM_212475.1
• NM_212476.1
• NM_212478.1
• NM_212482.1

1 mg/mL

One major band at 120kDa in SDS-PAGE;