Human Plasma Fibronectin Purified Protein
Description:
Human Plasma Fibronectin Purified Protein
Trade Name:
Chemicon (Millipore)
Qty/Pk:
1 mg
Product Overview:
Human fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plastic ware, glassware, and microcarrier beads.
Key Applications:
Cell Culture
Application Notes:
Immunochemical standard
Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts
Suggested Procedure for Coating Cell Cultureware
1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (μg/mL) of HFN. Recommended amount is 2-10 μg/cm2.
2.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.
3.Introduce the proper CO2 atmosphere, if required.
4.Add the calculated amount of HFN to each culture vessel.
5.Allow HFN to adsorb to the surface of the vessel for 5-20 minutes.
6.Remove residual balanced salt solution before proceeding with standard cell culture procedures
Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts
Suggested Procedure for Coating Cell Cultureware
1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (μg/mL) of HFN. Recommended amount is 2-10 μg/cm2.
2.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.
3.Introduce the proper CO2 atmosphere, if required.
4.Add the calculated amount of HFN to each culture vessel.
5.Allow HFN to adsorb to the surface of the vessel for 5-20 minutes.
6.Remove residual balanced salt solution before proceeding with standard cell culture procedures
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
UniProt Summary:
FUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity).
SIZE: 2386 amino acids; 262607 Da
SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1.
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
PTM: Sulfated.
SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.
SIZE: 2386 amino acids; 262607 Da
SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1.
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
PTM: Sulfated.
SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.
Species:
Human
Stem Cell Type:
- Human Embryonic Stem Cells
- Mesenchymal Stem Cells
- Neural Stem Cells
- Hematopoietic Stem Cells
- Epithelial Cells
- Pancreatic Stem Cells
- Induced Pluripotent Stem Cells
Stem Cell Workflow Stage:
- Maintenance & Expansion
- General Cell Culture
- Differentiation
Brand Family:
Chemicon
Source:
Human donor plasma.
Presentation:
Liquid in 150 mM NaCl, 10 mM sodium phosphate, pH 7.5, sterile filtered, containing no preservatives.
UniProt Number:
Storage Conditions:
Maintain at 2-8°C for up to 6 months from date of receipt. Do not freeze.
Cell Culture Reagent Type:
- Stem Cell Reagents
- Attachment Factors
Protein/Isoform Description:
Fibronectin (FN) exists in 3 forms: plasma FN, a soluble dimeric form that becomes incororated into the Fibrin meshwork of clots; cell-surface FN, oligomers transiently attached to the cell surface; and matrix FN, highly insoluble fibrils in the extracellular matrix. The 110 kDa cell-binding fragment of FN serves as a substrate for cells that express the α5β1 Integrin receptor and other Integrin receptors that recognize the Arg-Gly-Asp (RGD) sequence. It does not contain the Hep-2/CS1 region that is recognized by α4β1 Integrin.
Gene Symbol:
- FN1
- DKFZp686O13149
- DKFZp686H0342
- DKFZp686I1370
- MSF
- FN
- CIG
- DKFZp686F10164
- LETS
- FINC
Protein or Enzyme Type:
- Extracellular Matrix Proteins
- Purified Protein
Product Name:
Human Plasma Fibronectin Purified Protein
Entrez Gene Number:
Concentration:
1 mg/mL
Purity:
Approximately 95%, as determined by SDS-PAGE. A double band of 220 kDa is present under reduced conditions. hFN is purified by affinity chromatography on gelatin agarose, followed by chromatography on heparin-agarose. Plasma from donors has been screened, and shown to be negative for HIV, HTLV, Hepatitis B and C.