Ordering Information
Human Plasma Fibronectin Purified Protein
Description:
Human Plasma Fibronectin Purified Protein
Trade Name:
Chemicon (Millipore)
Product Overview:
Human fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plastic ware, glassware, and microcarrier beads.
Key Applications:
Cell Culture
Application Notes:
Immunochemical standard
Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts
Suggested Procedure for Coating Cell Cultureware
1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (μg/mL) of HFN. Recommended amount is 2-10 μg/cm2.
2.Reconstitute HFN at 1mg/mL and dilute to the desired concentration with physiological buffer.
3.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.
4. Introduce the proper CO2 atmosphere, if required.
5. Add the calculated amount of HFN to each culture vessel.
6. Allow HFN to adsorb to the surface of the vessel for 5-20 minutes.
7. Remove residual balanced salt solution before proceeding with standard cell culture procedures
Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts
Suggested Procedure for Coating Cell Cultureware
1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (μg/mL) of HFN. Recommended amount is 2-10 μg/cm2.
2.Reconstitute HFN at 1mg/mL and dilute to the desired concentration with physiological buffer.
3.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.
4. Introduce the proper CO2 atmosphere, if required.
5. Add the calculated amount of HFN to each culture vessel.
6. Allow HFN to adsorb to the surface of the vessel for 5-20 minutes.
7. Remove residual balanced salt solution before proceeding with standard cell culture procedures
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Stem Cell Type:
- Human Embryonic Stem Cells
- Mesenchymal Stem Cells
- Neural Stem Cells
- Hematopoietic Stem Cells
- Epithelial Cells
- Pancreatic Stem Cells
- Induced Pluripotent Stem Cells
Stem Cell Workflow Stage:
- Maintenance & Expansion
- General Cell Culture
- Differentiation
Source:
Human donor plasma.
Presentation:
Supplied in 5 individual vials. Liquid in 150 mM NaCl, 10 mM sodium phosphate, pH 7.5, sterile filtered, containing no preservatives.
Storage Conditions:
Maintain at 2-8°C for up to 6 months from date of receipt. Do not freeze.
Packaging:
5 x 1 mg
Cell Culture Reagent Type:
- Stem Cell Reagents
- Attachment Factors
Gene Symbol:
- FN1
- DKFZp686O13149
- DKFZp686H0342
- DKFZp686I1370
- MSF
- FN
- CIG
- DKFZp686F10164
- LETS
- FINC
Protein or Enzyme Type:
Extracellular Matrix Proteins
Concentration:
1 mg/mL
Purity:
Approximately 95%, as determined by SDS-PAGE. A double band of 220 kDa is present under reduced conditions. HFN is purified by affinity chromatography on gelatin agarose, followed by chromatography on heparin-agarose.
Protein Type:
Purified Protein