Product Images
Flow Cytometry Analysis:
Representative lot data.
Flow Cytometry analysis using Jurkat cells, stained with anti-MAPK1/2 antibody (Red), or...
Milli-Mark™ Anti-MAPK 1/2 (tERK), Alexa Fluor 647 Conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | Rabbit | AlexaFluor®647 | Polyclonal Antibody |
Description:
Milli-Mark™ Anti-MAPK 1/2 (tERK), Alexa Fluor 647 Conjugate
Trade Name:
Milli-Mark
Specificity:
Antibody recognizes MAPK1/2. C-terminus.
Molecular Weight:
43 kDa Calculated
Epitope:
C-terminus
Immunogen:
KLH-conjugated linear peptide corresponding to rat MAPK 1/2 at the C-terminus.
Background Information:
The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called MAPK1 and MAPK2, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or MAPK1/2. The MAPK1/2 signaling cascade has been shown to be a critical regulator of cell differentiation, cell physiology and neuronal function. Aberrant control of MAPK1/2 activity has been implicated in a variety of pathological conditions, including cancer and autoimmune diseases, and efficient study methods are in demand.
Species Reactivity:
Human
Species Reactivity Note:
Tested and passed on Human.
Control:
Jurkat cells
Quality Assurance:
Evaluated by flow cytometry using Jurkat cells
Purification Method:
Protein A purfied
Presentation:
Purified rabbit polyclonal antibody conjugated to Alexa Fluor 647 in PBS buffer with 15mg/mL BSA and 0.1% Sodium Azide
Storage Conditions:
Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- MAPK1
- p38
- ERK
- P42MAPK
- ERK2
- p40
- PRKM1
- p41mapk
- PRKM2
- MAPK2
- p42-MAPK
- p41
- ERK-2
- ERT1
Alternate Names:
- Extracellular signal-regulated kinase 1
- ERK-1
- Insulin-stimulated MAP2 kinase
- Mitogen-activated protein kinase 1
- MAP kinase 1
- MAPK 1
- p44-ERK1
- ERT2
- MAP kinase isoform p44
- p44
- MAPK
- Microtubule-associated protein 2 kinase
- MNK1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Flow Cytometry
Entrez Gene Summary:
Kinase involved in intracellular signalling; component of Mapk signalling pathway [RGD]
UniProt Summary:
FUNCTION: Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1. Phosphorylates heat shock factor protein 4 (HSF4) By similarity
Protein/Isoform Description:
MAP Kinases, or Erks, are Ser/Thr kinases which phosphorylate PXS/TP motifs in many different proteins, and are activated principally in response to mitogenic stimulation. MAP Kinases comprise MAP kinase 1/Erk1, or p44 MAP kinase, and MAP kinase 2/Erk2, or p42 MAP kinase. Both are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. No known mutations exist which can lead to constitutive activation of MAP kinases. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases.
Product Name:
Milli-Mark™ Anti-MAPK 1/2 (tERK), Alexa Fluor 647 Conjugate
Concentration:
0.5 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 tests
Format:
AlexaFluor®647
Host:
Rabbit