Product Images
Flow Cytometry Analysis:
Representative lot data.
FC analysis of HeLa cells stained with Anti-REST, APC conjugated. Cells were stained wi...
Milli-Mark™ Anti-REST-APC
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | FC | Rabbit | Purified | Polyclonal Antibody |
Description:
Milli-Mark™ Anti-REST-APC
Trade Name:
Milli-Mark
Specificity:
Antibody recognizes REST.
Immunogen:
Linear peptide corresponding to human REST.
Isotype:
IgG
Background Information:
NRSF (neuron-restrictive silencer factor, also designated XBR and REST for RE1-silencing factor) is a silencer protein that binds the DNA sequence element NRSE (neuron-restrictive silencer element). The binding of NRSF to the NRSE represses neuronal gene transcription in non-neuronal cells. Although NRSF is most highly expressed in non-neural tissues, it is also expressed in developing neurons and at low levels in the brain. NRSF contains nine zinc finger domains, but also exists as a C-terminally truncated form produced by alternative splicing. This variant, REST4, contains five of the zinc-finger domains and weakly binds DNA, yet is transported to the nucleus. NRSF associates with mSin3 and HDAC in ventricular myocytes, suggesting a role for NRSF outside the nervous system. Down-regulation of NRSF, which normally occurs upon neural differentiation, is necessary for the proper development of certain classes of neurons. NRSF is required to repress neuronal gene expression in vivo, in both extra-neural and undifferentiated neural tissue.
Species Reactivity:
- Human
- Mouse
- Rat
Species Reactivity Note:
Tested and passed on Human. Predicted on Mouse, Rat based on 100% sequence homology.
Control:
HeLa cells.
Quality Assurance:
Evaluated by flow cytometry using HeLa cells.
Purification Method:
Protein A Purified
Presentation:
Purified rabbit polyclonal IgG conjugated to APC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Storage Conditions:
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- REST
- NRSF
- XBR
Alternate Names:
- Neural-restrictive silencer factor
- RE1-silencing transcription factor
- X2 box repressor
- neuron restrictive silencer factor
- repressor binding to the X2 box
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Flow Cytometry
UniProt Summary:
FUNCTION: SwissProt: Q13127 # Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.
SIZE: 1097 amino acids; 121888 Da
SUBUNIT: Interacts with SIN3A, SIN3B and RCOR1.
SUBCELLULAR LOCATION: Nucleus (Probable).
TISSUE SPECIFICITY: Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary.
SIMILARITY: SwissProt: Q13127 ## Contains 9 C2H2-type zinc fingers.
SIZE: 1097 amino acids; 121888 Da
SUBUNIT: Interacts with SIN3A, SIN3B and RCOR1.
SUBCELLULAR LOCATION: Nucleus (Probable).
TISSUE SPECIFICITY: Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary.
SIMILARITY: SwissProt: Q13127 ## Contains 9 C2H2-type zinc fingers.
Format:
Purified
Host:
Rabbit
Protein/Isoform Description:
AKA NRSF, a DNA-binding transcriptional repressor that represses neuronal genes in non-neuronal lineages. It interacts with Co-REST, a co-repressor that recruits HDAC activity.
Product Name:
Milli-Mark™ Anti-REST-APC
Antibody Type:
Polyclonal Antibody