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  • : FCCH025103
  • : 25 Tests / Kit
  • Product Family Information

FlowCellect™ Bivariate Cell Cycle Kit for G2/M Analysis

Description:
FlowCellect™ Bivariate Cell Cycle Kit for G2/M Analysis
Trade Name:
FlowCellect
Qty/Pk:
25 Tests / Kit
Product Overview:
Millipore’s FlowCellect™ Bivariate Cell Cycle Kit for G2/M Analysis is designed to allow the researcher to easily identify, discriminate, and quantitate cells between the G2 and M-phases of the cell cycle. The kit can be used to perform bivariate analysis to measure cell cycle distributions by discrimination of cell cycle phases without the need of sophisticated software modules or algorithms. Having the ability to distinguish cells undergoing mitosis versus cells remaining in G2 phase of the cell cycle provides researchers with a powerful tool to closely monitor cell cycle activity at M-phase which can have a huge impact in studying diseases associated with cell proliferation, apoptosis, and cancer.

All FlowCellect kits are optimized on the bench-top guava® flow cytometry systems, which saves valuable time and sample volume. All cell cycle kits contain optimized fixation, permeabilization, wash, and flow buffers to provide researchers with a complete solution for bivariate analysis of total DNA content versus cell cycle phase markers. With the guava platform and FlowCellect kits, researchers can finally have an easy, reliable and fully validated solution to study complex cell cycle distribution analysis right in the comfort of their own lab.
Background Information:
Examination of the cell cycle has proven to be an accurate and effective method for assessing cell health by analyzing cell proliferation in great detail. Also recognized as the “Circle of Life”, the cell cycle can be divided into two distinct stages. The first stage is Interphase which consists of the G1, S, and G2 phases where cells are active, growing, and DNA replication takes place. The second is M-phase, also known as the “mitotic phase”, where cell division takes place. Researchers have employed various strategies to closely interrogate each of the critical steps at various phases in the cell cycle. For example, analysis of cells in S-phase will provide a direct measurement of newly synthesized DNA. Also, having the ability to discriminate cells between the G2 and M-phases can serve as a good indicator of cells undergoing mitosis. In all, a comprehensive understanding of cell cycle behavior can provide the researcher with useful information which will be important in interpreting the intrinsic nature of cell proliferation and assist in the development of anti-neoplastic agents.

Flow cytometric analysis of cell DNA content is widely used for the estimation of cell cycle phase distributions. However, the limitation in evaluating by only one marker (such as a DNA dye only) is that cells within each phase cannot accurately be determined without the use of software modules. Studying the cell cycle is now made easier by using a cell cycle phase-specific antibody plus a DNA dye in flow cytometry applications. Bivariate analysis using flow cytometry will not only reveal the distribution of cells in particular phases of the cycle, but will also allow the researcher to elucidate the molecular and functional mechanisms associated within the cell cycle.
Key Applications:
Flow Cytometry
Species Reactivity:
Human
Usage Statement:
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
  • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Quality Assurance:
Lot to lot in-process testing on all kit components
Components:
  • 1. 20X Anti-phospho-Histone H3 (Ser10) - Alexa Fluor 488 Conjugated Monoclonal Antibody: (Part No. CS204814) One vial containing 150 µL antibody.
  • 2. Propidium Iodide Solution: (Part No. CS204836) One vial containing 400 µL DNA dye.
  • 3. RNase A Reagent: (Part No. CS204821) One vial containing 25 µL.
  • 4. Fixation Buffer: (Part No. CS202122) One bottle containing 13 mL buffer.
  • 5. 10X Wash Buffer: (Part No. CS202123) One bottle containing 13 mL buffer.
  • 6. 5X Assay Buffer: (Part No. CS202124) One bottle containing 55 mL buffer.
  • 7.1X Permeabilization Buffer: (Part No. CS202127) One bottle containing 13 mL buffer.
Host:
Mouse
Storage Conditions:
4-8°C for antibodies and buffers
Detection Methods:
Fluorescent
Product Name:
FlowCellect™ Bivariate Cell Cycle Kit for G2/M Analysis
Alternate Names:
Flow Cellect
Antibody Type:
Monoclonal Antibody
Flow Cytometry Market:
Research