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Analyzed Dual Parameter Data: Dot plots depicting Jurkat cells stained using MitoLive Kit. Jurkat cells were uninduced (Plot A), Induced with 150 uM...
FlowCellect™ MitoLive Kit
Description:
FlowCellect MitoLive Kit
Trade Name:
FlowCellect
Qty/Pk:
100 Tests
Product Overview:
Millipore’s FlowCellect™ MitoLive Kit includes MitoSense Red, a fluorescent cationic dye that accumulates in the mitochondria and is responsive to mitochondrial potential changes and Calcein acetoxymethylester (calcein-AM) a non-fluorescent, cell permeant compound that is hydrolyzed by intracellular esterases into the fluorescent anion calcein and provides a measure of cellular vitality. The simultaneous use of the reagents allows researchers to obtain information on early and late apoptosis in one simple assay. MitoSense Red is excitable by a red laser and fluoresces maximally at 650 nm (Red2 channel fluorescence on the guava easyCyte 8HT). Uninduced cells with intact mitochondrial membrane potential demonstrate high Red2 fluorescence while cells which have impaired mitochondrial membrane potential depict lower Red2 fluorescence. The cell permeant dye, Calcein-AM included in the kit, enters all cells and if the cell is vital is hydrolyzed by intracellular esterases into the fluorescent anion calcein which are retained in cells with intact membranes. Healthy cells thus exhibit high green fluorescence. Cells in which the intracellular esterases are impacted on treatment or have compromised membranes demonstrate low green fluorescence. The assay kit allows the identification of 3 populations of cells; healthy live cells with intact membrane potential, live cells with impaired mitochondria and non-vital or dead cells with impaired mitochondria. The entire assay can be performed in approximately 30 min once cellular samples are ready.
Key Applications:
Flow Cytometry
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Components:
- MitoSense Red Dye
- Calcien-AM
- 10X Assay Buffer
- User Manual
Detection Methods:
Fluorescent
Product Name:
FlowCellect™ MitoLive Kit
Alternate Names:
FlowCellect
Flow Cytometry Software Type:
inCyte
Flow Cytometry Market:
- Bioprocess
- Research
Flow Cytometry Research Subcategory:
Early