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Analyzed Dual Parameter Data: Dot plots depicting Jurkat cells treated with multiple concentration of inducers and stained using Annexin Red Kit. Ju...
FlowCellect™ Annexin Red Kit
Description:
FlowCellect Annexin Red Kit
Trade Name:
FlowCellect
Qty/Pk:
100 Tests
Product Overview:
Millipore’s FlowCellect Annexin Red Kit provides a rapid and convenient assay to monitor early and late apotosis. The kit includes recombinant Annexin V conjugated to a red sensitive dye, CF647, to provide maximum sensitivity and 7-AAD, a cell impermeant dye which is an indicator of cell membrane structural integrity along with an assay buffer. 7-AAD is excluded from live, healthy cells and early apoptotic cells but stains late stage apoptotic and dead cells with red fluorescence by binding to nucleic acids in the cell. After staining cells with Flowcellect Annexin Red Kit, three populations of cells can be identified in this assay:
• Non-apoptotic cells: Annexin V(-) and 7-AAD(-)
• Early apoptotic cells: Annexin V(+) and 7-AAD(-)
• Late stage apoptotic or dead cells: Annexin V(+) and 7-AAD(+)
The kit permits users to pair the provided reagents with other reagents that fluoresce in the green and yellow channels of the flow cytometer. Sufficient reagents are provided for 100 tests. The kit includes all optimized fluorescently labeled reagents and buffers necessary for cell preparation and analysis.
• Non-apoptotic cells: Annexin V(-) and 7-AAD(-)
• Early apoptotic cells: Annexin V(+) and 7-AAD(-)
• Late stage apoptotic or dead cells: Annexin V(+) and 7-AAD(+)
The kit permits users to pair the provided reagents with other reagents that fluoresce in the green and yellow channels of the flow cytometer. Sufficient reagents are provided for 100 tests. The kit includes all optimized fluorescently labeled reagents and buffers necessary for cell preparation and analysis.
Key Applications:
Flow Cytometry
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Components:
- Annexin VCF647
- 7AAD
- 10X Assay Buffer
- User Manual
Detection Methods:
Fluorescent
Product Name:
FlowCellect™ Annexin Red Kit
Alternate Names:
FlowCellect
Flow Cytometry Software Type:
inCyte
Flow Cytometry Market:
- Bioprocess
- Research
Flow Cytometry Research Subcategory:
Early