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Analyzed Dual Parameter Data: Dot plot analysis Jurkat cells treated with different inducers and then stained using FlowCellect MitoStress Kit. Jurka...
FlowCellect™ MitoStress Kit
Description:
FlowCellect MitoStress Kit
Trade Name:
FlowCellect
Qty/Pk:
100 Tests
Product Overview:
Millipore’s FlowCellect™ MitoStress Kit includes MitoSOX Red, a live-cell permeant fluorogenic dye which targets the mitochondria and reacts with superoxide radicals and fluoresces yellow/red, and Annexin V conjugated to CF647 which binds to phosphatidylserine (PS) on the surface of apoptotic cells. The simultaneous use of these reagents allows researchers to obtain information on oxidative stress and apoptosis in one simple assay.
MitoSOX Red is excitable by a 488nm laser and fluoresces maximally at 580 nm (Yellow fluorescence on the guava easyCyte 8HT). Annexin V is calcium-dependent phospholipid binding protein with high affinity for PS, a membrane component normally localized to the internal face of the cell membrane. It is conjugated to CF647 which is excited by a red laser and emits at 665 nm (Red2 fluorescence on the guava easyCyte 8HT). Control cells depict no Red2 fluorescence while apoptotic cells exhibit positive Red2 fluorescence. The kit can thus distinguish multiple populations: 1) Live cells with little or no superoxide 2) Stressed cells with oxidized MitoSOX Red (indicator of accumulated superoxide) but no Annexin V staining 3) Stressed and Early Apoptotic cells with oxidized MitoSOX Red and Annexin V binding and 4) Apoptotic Cells with Annexin V binding only., The MitoStress kit rapidly provides information on correlation of mitochondrial superoxide stress with apoptosis using minimal cellular sample. The entire assay can be performed in less than 30 minutes, once cellular samples are ready.
MitoSOX Red is excitable by a 488nm laser and fluoresces maximally at 580 nm (Yellow fluorescence on the guava easyCyte 8HT). Annexin V is calcium-dependent phospholipid binding protein with high affinity for PS, a membrane component normally localized to the internal face of the cell membrane. It is conjugated to CF647 which is excited by a red laser and emits at 665 nm (Red2 fluorescence on the guava easyCyte 8HT). Control cells depict no Red2 fluorescence while apoptotic cells exhibit positive Red2 fluorescence. The kit can thus distinguish multiple populations: 1) Live cells with little or no superoxide 2) Stressed cells with oxidized MitoSOX Red (indicator of accumulated superoxide) but no Annexin V staining 3) Stressed and Early Apoptotic cells with oxidized MitoSOX Red and Annexin V binding and 4) Apoptotic Cells with Annexin V binding only., The MitoStress kit rapidly provides information on correlation of mitochondrial superoxide stress with apoptosis using minimal cellular sample. The entire assay can be performed in less than 30 minutes, once cellular samples are ready.
Key Applications:
Flow Cytometry
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Components:
- MitoSOX Red
- Annexin V CF647
- 10X Assay Buffer
- User Manual
Detection Methods:
Fluorescent
Product Name:
FlowCellect™ MitoStress Kit
Alternate Names:
FlowCellect
Flow Cytometry Software Type:
inCyte
Flow Cytometry Market:
- Bioprocess
- Research
Flow Cytometry Research Subcategory:
Early