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  • : FCCS025108
  • : 25 Tests / Kit
  • Product Family Information

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Multi-color dot blot analysis of Bcl-2 expression and Bcl-2 phosphorylation at serine 70. Bcl-2 expression remains constant but pBcl-2(S70) phosphoryl...

FlowCellect™ Bcl-2 Activation Dual Detection Kit

Description:
FlowCellect™ Bcl-2 Activation Dual Detection Kit
Trade Name:
FlowCellect
Qty/Pk:
25 Tests / Kit
Product Overview:
Millipore’s FlowCellect™ Bcl-2 Activation Dual Detection kit includes two directly conjugated antibodies: Anti-Bcl-2-Alexa Fluor® 488 and Anti-pBcl-2 (Serine 70)-PE, along with optimized fixation, permeabilization, wash, and assay buffers necessary for cell preparation and analysis. Millipore’s FlowCellect™ Activation Dual Detection Kit contains sufficient reagents for 25 2-color samples. Detailed assay instructions are included to assist in your analysis and to ensure that the correct cell concentration is obtained during acquisition of sample data.

Millipore’s FlowCellect™ Bcl-2 Activation Dual Detection Kit is designed to enable a researcher a quick and easy way to detect total Bcl-2 expression as well as the phosphorylation of Bcl-2 at serine 70 simultaneously. Millipore’s FlowCellect™ Activation Dual Dectection Kit was developed and tested using Jurkat cells treated with staurosporine, but the kit can be used with other human cell lines to determine the effect of other chemical reagents on Bcl-2 expression and phosphorylation.
Background Information:
The signaling pathways that regulate apoptosis are important in understanding tissue homeostasis, the development of the immune system, and therapies for cancer. Bcl-2 is a member of the Bcl-2 family that regulates apoptosis by regulating the proteins that control mitochondrial membrane permeability.

The Bcl-2 family consists of two protein sub-groups, the anti-apoptotic proteins and the pro-apototic proteins (3). Bcl-2 is an anti-apoptotic family member that binds to pro-apoptotic family members, such as Bax to sequester them and inhibit insertion into the mitochondrial membrane. When cells receive an apoptotic signal, Bcl-2 releases Bax, allowing Bax to form a complex on the mitochondrial membrane that releases cytochrome C into the cytoplasm leading to caspase activation and cell death.

Bcl-2 has been shown to be regulated at the level of phosphorylation by JNK at a multi-site phosphorylation loop which includes serine 70. Phosphorylation of Bcl-2 at Serine 70 has been shown to be important in both apoptosis and in autophagy.
Key Applications:
Flow Cytometry
Species Reactivity:
Human
Usage Statement:
  • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Conditions:
4-8°C for antibodies and buffers
Product Name:
FlowCellect™ Bcl-2 Activation Dual Detection Kit
Alternate Names:
Flow Cellect
Flow Cytometry Software Type:
ExpressPro & inCyte
Antibody Type:
Monoclonal Antibody
Flow Cytometry Market:
Research
Quality Assurance:
Lot to lot in-process testing on all kit components
Components:
  • 1. 20X Anti-Bcl-2 Alexa Fluor® 488 Antibody (Part No. CS205126) One vial containing 150 µL antibody.
  • 2. 20X Anti-pBcl-2(Ser70) PE Antibody (Part No. CS205125) One vial containing 150 µL antibody.
  • 3. Fixation Buffer: (Part No. CS202122) One bottle containing 13 mL.
  • 4. 10X Wash Buffer: (Part No. CS202123) One bottle containing 13 mL.
  • 5. 5X Assay Buffer: (Part No. CS202124) One 55 mL bottle
  • 6. 1X Permeabilization Buffer: (Part No. CS203284) One bottle containing 13 mL.
Host:
Mouse
Detection Methods:
Fluorescent