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Ordering Information

  • : FCCS025132
  • : 25 Tests / Kit
  • Product Family Information

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Multi-color dot blot analysis of P38 and ATF2 phosphorylation upon stimulation with anisomycin treatment. Untreated Jurkat cells (A) and Jurkat cells...

FlowCellect™ p38 Stress Pathway Activation Detection Kit

Description:
FlowCellect™ p38 Stress Pathway Activation Detection Kit
Trade Name:
FlowCellect
Qty/Pk:
25 Tests / Kit
Product Overview:
Millipore’s FlowCellect™ p38 Stress Pathway Activation Detection Kit is designed to enable a researcher a quick and easy way to detect the phosphorylation state of P38α and ATF2 by flow cytometry. Millipore’s FlowCellect™ p38 Stress Pathway Activation Kit was developed and tested using the activating reagent, anisomycin, in Jurkat cells but the kit can be used with other human cell lines to determine the effect of stress stimuli and chemical reagents that can induce P38 pathway signaling.
Millipore’s FlowCellect™ p38 Stress Pathway Activation Detection Kit contains sufficient reagents for 25 2-color tests. The kit includes two optimized fluorescent labeled antibodies and buffers necessary for cell preparation and analysis. Detailed assay instructions are included to assist in your analysis and to ensure that the correct cell concentration is obtained during acquisition of sample data.
Background Information:
p38 is a member of the mitogen activated kinase family that responds to stress stimuli, such as inflammatory cytokines, ultraviolet irradiation, heat shock, and osmotic shock. p38 is involved in cell differentiation and apoptosis. More recently, P38 has been shown to have a role in cell cycle check points and DNA damage pathways. The upstream kinases, MKK3, MKK6 and SEK activate p38α by phosphorylation at Thr180 and Tyr182.
Once activated, p38α MAP kinase has been shown to phosphorylate and activate a number of proteins including MAPKAP kinase 2 and the transcription factors ATF-2, MEF2 and MSK1.
Key Applications:
Flow Cytometry
Species Reactivity:
Human
Usage Statement:
  • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Conditions:
4-8°C for antibodies and buffers
Product Name:
FlowCellect™ p38 Stress Pathway Activation Detection Kit
Alternate Names:
Flow Cellect
Flow Cytometry Software Type:
ExpressPro & inCyte
Antibody Type:
Monoclonal Antibody
Flow Cytometry Market:
Research
Quality Assurance:
Lot to lot in-process testing on all kit components
Components:
  • 1. 20X Anti-pP38(T180/Y182) AlexaFluor®488 Antibody: (Part No. CS205783) 1 vial containing µL.
  • 2. 20X Anti-pATF2(T69/71) AlexaFluor®647 Antibody: (Part No.CS205784) 1 vial containing 150 µL.
  • 3. Fixation Buffer: (Part No. CS202122) One bottle containing 13 mL.
  • 4. 10X Wash Buffer: (Part No. CS202123) One bottle containing 13 mL.
  • 5. 5X Assay Buffer: (Part No. CS202124) One 55 mL bottle
  • 6. 1X Permeabilization Buffer: (Part No. CS203284) One bottle containing 13 mL.
Host:
Mouse
Detection Methods:
Fluorescent