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Dual Parameter Analysis of Total and Phospho STAT1 on A431Cells (A) Unstimulated U937 cells stained with total and phospho STAT1. 97% of the cells ar...
FlowCellect™ STAT1 Activation Dual Detection Kit
Description:
FlowCellect™ STAT1 Activation Dual Detection Kit
Trade Name:
FlowCellect
Qty/Pk:
25 Tests / Kit
Product Overview:
Millipore’s FlowCellect™ STAT1 Activation Dual Detection kit includes two directly conjugated antibodies, a phospho-specific Anti-phospho-STAT1 (Tyr701)-Alexa Fluor® 488 and Anti-STAT1-PerCP. This two color flow cytometry kit is designed to detect the extent of STAT1 pathway activation by measuring the STAT1 phosphorylation relative to the total STAT1 expression in any given cell population.
The levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of STAT1 activation after stimulation. Moreover, simultaneous measurement of both total and phospho-STAT1 confirms target specificity of the phosphorylation event. Together, a total and phospho antibody duo performed in multiplex provides an enhanced and more reliable detection of the phospho: total ratio within a mixed population.
The levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of STAT1 activation after stimulation. Moreover, simultaneous measurement of both total and phospho-STAT1 confirms target specificity of the phosphorylation event. Together, a total and phospho antibody duo performed in multiplex provides an enhanced and more reliable detection of the phospho: total ratio within a mixed population.
Background Information:
STATs are transcription factors that can be phosphorylated normally by a cell surface receptor such as cytokine receptors, followed by dimerization and nuclear translocation where they bind to DNA and activate transcription of their target genes. Signal Transducer and Activator of Transcription 1 (STAT1) is involved in cellular immunity and cell proliferation. Its activation has been detected in response to viral as well as parasitic infections.
One interesting aspect of STAT1 activation is its role in antitumor biology. Macrophage stimulation with interferon gamma induces direct antimicrobial and antitumor mechanisms as well as upregulating antigen processing and presentation pathways. It has been shown that oncogenesis and tumor progression are supported by alterations in cell signaling. Using flow cytometry, it is now possible to track and analyze signaling events in individual cancer cells. Single cell resolution of signaling molecules allows for a greater understanding of the elaborate processes that occur when cells transform and begin replicating uncontrollably.
One interesting aspect of STAT1 activation is its role in antitumor biology. Macrophage stimulation with interferon gamma induces direct antimicrobial and antitumor mechanisms as well as upregulating antigen processing and presentation pathways. It has been shown that oncogenesis and tumor progression are supported by alterations in cell signaling. Using flow cytometry, it is now possible to track and analyze signaling events in individual cancer cells. Single cell resolution of signaling molecules allows for a greater understanding of the elaborate processes that occur when cells transform and begin replicating uncontrollably.
Key Applications:
Flow Cytometry
Species Reactivity:
Human
Usage Statement:
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Quality Assurance:
Lot to lot in-process testing on all kit components
Components:
- 1. 20X Anti-phospho-STAT1(Tyr701) Alexa Fluor® 488: (Part No. CS206484) One vial containing 150 µL.
- 2. 20X Anti-STAT1-PerCP: (Part No. CS206483) One vial containing 150 µL.
- 3. Fixation Buffer: (Part No. CS202122) One bottle containing 13 mL.
- 4. 10X Wash Buffer: (Part No. CS202123) One bottle containing 13 mL.
- 5. 5X Assay Buffer: (Part No. CS202124) One 55 mL bottle
- 6. 1X Permeabilization Buffer: (Part No. CS203284) One bottle containing 13 mL.
Host:
Mouse
Storage Conditions:
4-8°C for antibodies and buffers
Detection Methods:
Fluorescent
Product Name:
FlowCellect™ STAT1 Activation Dual Detection Kit
Alternate Names:
Flow Cellect
Flow Cytometry Software Type:
ExpressPro & inCyte
Antibody Type:
Monoclonal Antibody
Flow Cytometry Market:
Research