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  • : FCMAB100P
  • : 100 tests
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Flow Cytometry:
Representative lot data.
Flow Cytometry analysis using anti-phospho-ERK1/2 (Thr202/Tyr204, Thr185/Tyr187). Jurkat cells wer...

Flow Cytometry
Antibody dilution for cellular staining:
• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS...

Milli-Mark™ Phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187) -PE

Species Reactivity Key Applications Host Format Antibody Type
H, M, R FC, WB Rabbit PE Polyclonal Antibody
Description:
Milli-Mark Phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187)-PE
Trade Name:
Milli-Mark
Specificity:
Antibody recognizes Recognizes Erk 1 & 2 only when dually phosphorylated on its TxY activation motif.
Molecular Weight:
42kDa and 44kDa Observed
Epitope:
Phosphorylated Thr185/Phosphorylated Tyr187
Immunogen:
Phosphorylated recombinant peptide corresponding to amino acids surrounding the pTEpY motif in the activation loop of human phospho-Erk1/2, in which the Thr and Tyr residues are phosphorylated
Modifications:
Phosphorylation
Clone:
AW39R
Isotype:
IgG
Background Information:
Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as Erk1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator of the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs), GPCR, and/or integrin stimulation. This activates the Ras-Raf-MEK-Erk pathway that results in the phosphorylation/activation of Erk1/2 (p44/p42) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 & Erk2, respectively).
Species Reactivity:
  • Human
  • Mouse
  • Rat
Species Reactivity Note:
Tested and passed on Human. based on 100% sequence homology.
Control:
Jurkat Cells
Quality Assurance:
Evaluated by Flow Cytometry with Jurkat cells.
Purification Method:
Protein A purfied
Presentation:
Purified Rabbit monoclonal supernatant conjugated to phycoerythrin in buffer containing PBS with 0.05% sodium azide
Storage Conditions:
Stable for 6 months at 2-8°C from date of receipt. Protect from light.
UniProt Number:
P28482
Entrez Gene Number:
NP_002736.3
Gene Symbol:
Erk1
Erk2
p44-MAPK
p42-MAPK
MAPK2
PRKM2
p41mapk
PRKM1
p44
ERK2
P42MAPK
ERK
p42
MAPK1
ERT1
ERT2
Mitogen-activated protein kinase
MAP Kinase 2
MAP Kianse 1
MAPK
Alternate Names:
Erk1
Erk2
p44-MAPK
p42-MAPK
MAPK2
PRKM2
p41mapk
PRKM1
p44
ERK2
P42MAPK
ERK
p42
MAPK1
ERT1
ERT2
Mitogen-activated protein kinase
MAP Kinase 2
MAP Kianse 1
MAPK
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • Flow Cytometry
  • Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
UniProt Summary:
FUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
COFACTOR: Magnesium (By similarity).
SIZE: 360 amino acids; 41390 Da
SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.
DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
Product Name:
Milli-Mark™ Phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187) -PE
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 tests
Format:
PE
Host:
Rabbit

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