Ordering Information
- : FCMAB101A4
- : 100 tests
Product Images
Flow Cytometry:
Representative lot data.
Flow Cytometry analysis using anti-BrdU. Jurkat cells were treated with 10 uM Camptothecin for 4 ...
Flow Cytometry
Antibody dilution for cellular staining:
• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS...
Milli-Mark™ Anti-BrdU, clone BU-1 Alexa Fluor® 488 conjugated
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, ICC | Mouse | AlexaFluor®488 | Monoclonal Antibody |
Description:
Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor® 488 conjugated | FCMAB101A4
Promotional Text:
Trade Name:
Milli-Mark
Specificity:
Specifically binds to bromodeoxyuridine (crossreacts with iodouridine 10%). Does not crossreact with fluorodeoxyuridine, nor with any endogenous cellular components such as thymidine or uridine.
Molecular Weight:
none given
Immunogen:
Bromodeoxyuridine
Clone:
Bu-1
Isotype:
IgG2a
Background Information:
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. This antibody does not require pretreatment of cells with strong DNA denaturants in order for the antibody to recognize incorporated BrdU. The antibody's special ability is thought to be due to the presence of DNAse in the antibody solution arising from the mycoplasma infecting the original hybridoma line {Gonchoroff, 1985}.
Species Reactivity:
Human
Species Reactivity Note:
Tested on Human. Predicted to react with all species.
Control:
After incorporation of BrdU, all DNA containing species
Quality Assurance:
Evaluated by flow cytometry with Jurkat Cells.
Purification Method:
Protein G purfied
Presentation:
Presentation needs to say: Purified mouse monoclonal IgG2a supernatant conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA. Possible mycoplasma risk contained within this product. Use appropriate measures to prevent cell line infection/transmission.
Storage Conditions:
Stable for 6 months at 2-8°C from date of receipt. Protect from light.
Gene Symbol:
BrdU
Alternate Names:
BrdU
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Flow Cytometry
- Immunocytochemistry
Product Name:
Milli-Mark™ Anti-BrdU, clone BU-1 Alexa Fluor® 488 conjugated
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 tests
Format:
AlexaFluor®488
Host:
Mouse