Ordering Information
- : FCMAB102P
- : 100 tests
Product Images
Flow Cytometry:
Representative lot data.
Flow Cytometry analysis using anti-Cyclin B1. Jurkat cells were treated with 100 uM Nocodazole ov...
Flow Cytometry
Antibody dilution for cellular staining:
• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS...
Milli-Mark™ Anti-Cyclin B1-PE, clone GNS3 (8A5D12)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IP, FC | Mouse | Phycoerythrin | Monoclonal Antibody |
Description:
Milli-Mark Anti-Cyclin B1-PE Antibody, clone GNS3 (8A5D12) | FCMAB102P
Promotional Text:
Trade Name:
Milli-Mark
Specificity:
This antibody is specific for human cyclin B1, MW 58 kDa. Does not cross-react with cyclin A or cyclin D.
Molecular Weight:
58 kDa Observed
Epitope:
Unknown
Immunogen:
Recombinant human cyclin B1
Clone:
GNS3 (8A5D12)
Isotype:
IgG
Background Information:
Regulation of cell cycle progression in eukaryotic cells depends on the expression of cyclin proteins. These proteins are the regulatory subunits of the cell cycle-dependent kinases that are responsible for the phosphorylation of several cellular targets. The B cyclins, when combined with p34cdc2, a catalytic subunit of the cell cycle dependent kinase, form an enzyme that is active at the transition to the G2 phase, attaining maximum level at M phase. At that phase, the complex associates with condensed chromosomes in prophase and metaphase and is degraded at the metaphase/anaphase transition.
Anti-Cyclin B1 may be used for the detection of cyclin B1 expression and for measuring relative differences in cyclin B1 level as a function of cell cycle phase.
Anti-Cyclin B1 may be used for the detection of cyclin B1 expression and for measuring relative differences in cyclin B1 level as a function of cell cycle phase.
Species Reactivity:
- Human
- Mouse
Species Reactivity Note:
Tested and passed on Human. In addition to human, weak species cross-reactivity was observed with mouse.
Control:
Jurkat Cells
Quality Assurance:
Evaluated by Flow Cytometry on Jurkat cells.
Purification Method:
Protein G purfied
Presentation:
Protein G purified mouse IgGs conjugated to phycoerythrin in storage buffer PBS, 15mg/mL BSA and 0.1% sodium azide with out Glycerol.
Storage Conditions:
Maintain refrigerated at 2-8oC in undiluted aliquots for up to 6 months from date of receipt. Protect from light.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
G2/mitotic-specific cyclin B1
cyclin B1
cyclin B1
Alternate Names:
G2/mitotic-specific cyclin B1
cyclin B1
cyclin B1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunoprecipitation
- Flow Cytometry
Entrez Gene Summary:
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
UniProt Summary:
FUNCTION: SwissProt: P14635 # Essential for the control of the cell cycle at the G2/M (mitosis) transition.
SIZE: 433 amino acids; 48337 Da
SUBUNIT: Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Centrosome.
DEVELOPMENTAL STAGE: Accumulates steadily during G2 and is abruptly destroyed at mitosis.
PTM: Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
SIMILARITY: SwissProt: P14635 ## Belongs to the cyclin family. Cyclin AB subfamily.
SIZE: 433 amino acids; 48337 Da
SUBUNIT: Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Centrosome.
DEVELOPMENTAL STAGE: Accumulates steadily during G2 and is abruptly destroyed at mitosis.
PTM: Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
SIMILARITY: SwissProt: P14635 ## Belongs to the cyclin family. Cyclin AB subfamily.
Product Name:
Milli-Mark™ Anti-Cyclin B1-PE, clone GNS3 (8A5D12)
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 tests
Format:
Phycoerythrin
Host:
Mouse