Ordering Information
- : FCMAB105P
- : 100 tests
Product Images
Flow Cytometry:
Representative lot data.
Flow cytometry analysis using anti-pSTAT5A/B (Y694/Y699)-PE. U937 cells were treated with GM-CSF ...
Flow Cytometry
Antibody dilution for cellular staining:
• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS...
Milli-Mark™ Anti-phospho-STAT5A/B (Tyr694/699), clone 8-5-2 PE conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, B, Sh | WB, FC | Mouse | Phycoerythrin | Monoclonal Antibody |
Description:
Anti-phospho-STAT5A/B (Tyr694/699), clone 8-5-2 PE conjugate
Specificity:
Specific for the phosphorylated tyrosine residue 694 of STAT5A (94kDa) and 699 of STAT5B (92kDa).
Molecular Weight:
94 / 92 kDa Calculated STAT5A (94 kDa) and STAT5B (92 kDa)
Epitope:
Phosphorylated Tyr694/699
Immunogen:
KLH-conjugated, synthetic phosphopeptide corresponding to amino acids 689-700 of STAT5A and 694-705 of STAT5B.
Modifications:
Phosphorylation
Clone:
8-5-2
Isotype:
IgG1
Background Information:
STAT (Signal Transducers and Activators of Transcription) are a family of conserved transcription factors that transduce high-fidelity signals for the cytokine family of ligands and receptors as the principle substrates of JAK kinases (1,2). In resting cells, the STATs exist in an inactive state in the cytoplasm until activated. Activators include cytokines, growth factors, and some peptides. These activate JAK kinases, which when activated, bind to the STAT SH2 (Src homology domain-2) domain resulting in phosphorylation of STAT (1). Upon activation, STAT1, -3, -4, -5A, and -5B all form homodimers. STAT1 and STAT2, as well as STAT1 and STAT3 form heterodimers, and several STATs form tetramers, depending on the variables of the activating ligand (1). After dimerization, the STATs accumulate in the nucleus by way of nuclear transport (1). STAT5A and STAT5B appear to be most important to the function of growth hormone and prolactin and play critical roles in milk production (1,2,3). The phosphorylation of Tyrosine 694/699 is a hallmark of activated STAT5.
Species Reactivity:
- Human
- Mouse
- Bovine
- Sheep
Species Reactivity Note:
Tested and passed on Human. Predicted to react on Mouse, Bovine, Sheep based on 100% sequence homology.
Control:
U397 Cells
Quality Assurance:
Evaluated by Flow Cytometry with U937 Cells.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG conjugated to phycoerythrin in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Storage Conditions:
Maintain refrigerated at 2-8oC in undiluted aliquots for up to 6 months from date of receipt. Protect from light.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
STAT5A
MGF
STAT5
MGF
STAT5
Alternate Names:
signal transducer and activator of transcription 5A
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Flow Cytometry
Entrez Gene Summary:
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL. ...
UniProt Summary:
FUNCTION: Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
SUBUNIT STURCTURE: Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 By similarity. Interacts with NCOA1, NMI and SOCS7.
SUBCELLULAR LOCATION: CytoplasmBy similarity. NucleusBy similarity. Note= Translocated into the nucleus in response to phosphorylation By similarity.
PTM: >Tyrosine phosphorylated By similarity.
INVOLVEMENT IN DISEASE: Defects in STAT5B are the cause of Laron type dwarfism II (LTD2) [MIM:245590]; also known as Laron syndrome type II or Laron syndrome due to a post-receptor defect. The phenotypic features are consistent with growth hormone deficiency in the presence of normal to elevated circulating concentrations of growth hormone, and resistance to hexogeneous hormone therapy.
SEQUENCE SIMILARITIES: Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
SUBUNIT STURCTURE: Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 By similarity. Interacts with NCOA1, NMI and SOCS7.
SUBCELLULAR LOCATION: CytoplasmBy similarity. NucleusBy similarity. Note= Translocated into the nucleus in response to phosphorylation By similarity.
PTM: >Tyrosine phosphorylated By similarity.
INVOLVEMENT IN DISEASE: Defects in STAT5B are the cause of Laron type dwarfism II (LTD2) [MIM:245590]; also known as Laron syndrome type II or Laron syndrome due to a post-receptor defect. The phenotypic features are consistent with growth hormone deficiency in the presence of normal to elevated circulating concentrations of growth hormone, and resistance to hexogeneous hormone therapy.
SEQUENCE SIMILARITIES: Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
Product Name:
Milli-Mark™ Anti-phospho-STAT5A/B (Tyr694/699), clone 8-5-2 PE conjugate
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 tests
Format:
Phycoerythrin
Host:
Mouse
Long Description:
Signal Transducer and Activators of Transcription