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Flow Cytometry: Representative lot data.Flow cytometry analysis using anti-SOX-2-FITC. Rat neural stem cells were stained with either Isotype control ...
Milli-Mark™ Anti-SOX-2, clone 6G1.2
FITC conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | FC | Mouse | FITC | Monoclonal Antibody |
Description:
Anti-SOX-2, clone 6G1.2, FITC conjugate
Specificity:
SOX-2
Molecular Weight:
~34 kDa
Immunogen:
Recombinant GST Fusion protein.
Clone:
6G1.2
Isotype:
IgG2b
Background Information:
Sox-2 plays a key role in embryonic stem cell development, and has been shown to help stem cells maintain a pluripotent potential. It has also been shown to be associated with uncommitted dividing stem cell and precursor cells of the developing CNS.
Species Reactivity:
- Human
- Mouse
- Rat
Species Reactivity Note:
Tested and passed on Human. based on 100% sequence homology. Human and Rat
Application Notes:
Flow Cytometry:
Antibody dilution for cellular staining:
• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
• Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
• 5 µL for Guava Flow Cytometer
• 10 µL for other Flow Cytometry instruments
Antibody dilution for cellular staining:
• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
• Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
• 5 µL for Guava Flow Cytometer
• 10 µL for other Flow Cytometry instruments
| Working Solution | Cells / test | Total Reaction Volume |
|---|---|---|
| 5 μl | 5 x 105 Cells / 95 μl PBS | 100 μl |
| 10 μl | 1 x 106 Cells / 90 μl PBS | 100 μl |
Control:
Rat Neural Stem Cells.
Quality Assurance:
Evaluated by flow cytometry on undifferentiated adult Rat hippocampal neural stem cells SCR022.
Optimal working dilutions must be determined by the end user.
Optimal working dilutions must be determined by the end user.
Purification Method:
Protein A purfied
Presentation:
Purified mouse monoclonal IgG2b conjugated to FITC in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.
Storage Conditions:
Maintain refrigerated at 2-8oC protected from light in undiluted aliquots for up to 6 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- ANOP3
- MCOPS3
- MGC2413
Alternate Names:
- SRY (sex determining region Y)-box 2
- SRY-related HMG-box gene 2
- sex-determining region Y-box 2
- transcription factor SOX2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Flow Cytometry
Entrez Gene Summary:
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate.The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript
(SOX2OT).
(SOX2OT).
UniProt Summary:
FUNCTION: Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency By similarity.
SUBCELLULAR LOCATION: Nucleus.
PTM: Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation By similarity.
INVOLVEMENT IN DISEASE: Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
SEQUENCE SIMILARITIES: Contains 1 HMG box DNA-binding domain.
SUBCELLULAR LOCATION: Nucleus.
PTM: Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation By similarity.
INVOLVEMENT IN DISEASE: Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
SEQUENCE SIMILARITIES: Contains 1 HMG box DNA-binding domain.
Product Name:
Milli-Mark™ Anti-SOX-2, clone 6G1.2
FITC conjugate
FITC conjugate
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 tests
Format:
FITC
Host:
Mouse