Ordering Information
- : FCMAB113A4
- : 100 tests
Product Images
Flow Cytometry:
Representative lot data
Fixed and permeabilized 2102Ep cells were stained with anti-hOCT4-Alexa Fluor® 488(green histogram) ...
Representative lot data.
Immunocytochemical staining of H9 human ES cells with anti-Oct-4, clone 10H11.2, Alexa Fluor® 488 conjugate (Cat. No. FC...
Milli-Mark™ Anti-Oct-4, clone 10H11.2
Alexa Fluor® 488 conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IC | Mouse | AlexaFluor®488 | Monoclonal Antibody |
Description:
Anti-Oct-4, clone 10H11.2, Alexa Fluor® 488 conjugate
Specificity:
Reacts with human Oct-4.
Molecular Weight:
~44 kDa
Immunogen:
Recombinant GST fusion protein, human Oct-4 (aa 1-134).
Clone:
10H11.2
Isotype:
IgG1
Background Information:
Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent embryonic stem (ES) and embryonic carcinoma (EC) cells express Oct-4. Additionally, murine embryonic germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
Species Reactivity:
Human
Application Notes:
Flow Cytometry:
Antibody dilution for cellular staining:
• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
• Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
• 5 µL for Guava Flow Cytometer
• 10 µL for other Flow Cytometry instruments
Antibody dilution for cellular staining:
• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
• Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
• 5 µL for Guava Flow Cytometer
• 10 µL for other Flow Cytometry instruments
| Working Solution | Cells / test | Total Reaction Volume |
|---|---|---|
| 5 μl | 5 x 105 Cells / 95 μl PBS | 100 μl |
| 10 μl | 1 x 106 Cells / 90 μl PBS | 100 μl |
Control:
Human embryonic stem cells
Quality Assurance:
Evaluated by Flow Cytometry in 2102Ep Cells.
Presentation:
Purified mouse monoclonal IgG1 conjugated to Alexa Flour® 488 in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.
Storage Conditions:
Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- MGC22487
- OCT3
- OCT4
- OTF3
- OTF4
- OTTHUMP00000029292
- Oct-3
- Oct-4
- Oct4
Alternate Names:
- Octamer-binding transcription factor 3
- POU class 5 homeobox 1
- POU domain class 5, transcription factor 1
- POU domain, class 5, transcription factor 1
- POU-type homeodomain-containing DNA-binding protein
- octamer-binding transcription factor-3
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Flow Cytometry
- Immunocytochemistry
UniProt Summary:
FUNCTION:Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency By similarity.
SUBUNIT STRUCTURE:Interacts with UBE2I By similarity. Interacts with PKM2.
SUBCELLULAR LOCATION: Nucleus. Note: Expressed in a diffuse and slightly punctuate pattern.
TISSUE SPECIFICITY: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
INDUCTION: Transcriptional activity is positively regulated by PKM2.
DOMAIN: The POU-specific domain mediates interaction with PKM2.
PTM: Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression By similarity.
MISCELLANEOUS: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.
SEQUENCE SIMILARITIES:Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.Contains 1 POU-specific domain.
SUBUNIT STRUCTURE:Interacts with UBE2I By similarity. Interacts with PKM2.
SUBCELLULAR LOCATION: Nucleus. Note: Expressed in a diffuse and slightly punctuate pattern.
TISSUE SPECIFICITY: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
INDUCTION: Transcriptional activity is positively regulated by PKM2.
DOMAIN: The POU-specific domain mediates interaction with PKM2.
PTM: Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression By similarity.
MISCELLANEOUS: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.
SEQUENCE SIMILARITIES:Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.Contains 1 POU-specific domain.
Product Name:
Milli-Mark™ Anti-Oct-4, clone 10H11.2
Alexa Fluor® 488 conjugate
Alexa Fluor® 488 conjugate
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 tests
Format:
AlexaFluor®488
Host:
Mouse