Product Images
Flow Cytometry Analysis:
Representative lot data.
FACS analysis using NIH/3T3 cells, stained with anti-c-Jun antibody (green), or Sigma #F6...
Milli-Mark™ Anti-c-Jun, clone 6E4, FITC Conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | FC | Mouse | FITC | Monoclonal Antibody |
Description:
Milli-Mark™ Anti-c-Jun Antibody, clone 6E4, FITC Conjugate | FCMAB122F
Promotional Text:
Trade Name:
Milli-Mark
Specificity:
Antibody recognizes c-Jun. .
Molecular Weight:
36-43kDa Observed
Immunogen:
GST-tagged recombinant protein corresponding to c-Jun.
Clone:
6E4
Isotype:
IgG1κ
Background Information:
c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.
Species Reactivity:
- Human
- Mouse
- Rat
Species Reactivity Note:
Tested and passed on Mouse. Unconjugated antibody shown to work in Human, Mouse and Rat using ICC.
Application Notes:
| Working Solution | Cells / test | Total Reaction Volume |
|---|---|---|
| 5 μl | 5 x 105 Cells / 95 μl PBS | 100 μl |
| 10 μl | 1 x 106 Cells / 90 μl PBS | 100 μl |
Control:
NIH/3T3 cells
Quality Assurance:
Evaluated by flow cytometry using NIH/3T3
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Storage Conditions:
Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
EC 2.7.11.24
JNK
JNK-46
JNK1
JNK1A2
JNK21B1/2
OTTHUMP00000019552
OTTHUMP00000019555
OTTHUMP00000019556
OTTHUMP00000019558
PRKM8
SAPK1
JNK
JNK-46
JNK1
JNK1A2
JNK21B1/2
OTTHUMP00000019552
OTTHUMP00000019555
OTTHUMP00000019556
OTTHUMP00000019558
PRKM8
SAPK1
Alternate Names:
JNK1 alpha protein kinase
JNK1 beta protein kinase
JUN N-terminal kinase
Stress-activated protein kinase JNK1
c-Jun N-terminal kinase 1
mitogen-activated protein kinase 8
mitogen-activated protein kinase 8 isoform JNK1 alpha1
mitogen-activated protein kinase 8 isoform JNK1 beta2
protein kinase JNK1
JNK1 beta protein kinase
JUN N-terminal kinase
Stress-activated protein kinase JNK1
c-Jun N-terminal kinase 1
mitogen-activated protein kinase 8
mitogen-activated protein kinase 8 isoform JNK1 alpha1
mitogen-activated protein kinase 8 isoform JNK1 beta2
protein kinase JNK1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Flow Cytometry
Entrez Gene Summary:
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involvedin both translocations and deletions in human malignancies.
UniProt Summary:
FUNCTION: Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells By similarity. Phosphorylates heat shock factor protein 4 (HSF4).
Product Name:
Milli-Mark™ Anti-c-Jun, clone 6E4, FITC Conjugate
Concentration:
0.5 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 tests
Format:
FITC
Host:
Mouse