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Flow Cytometry Analysis:
Representative lot data.
FACS analysis of mouse embryonic stem cells stained with anti-mOCT4 AlexaFluor®488 (gree...
Milli-Mark™ Anti-mOCT4, clone 7F9.2, Alexa Fluor® 488 Conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | FC | Mouse | AlexaFluor®488 | Monoclonal Antibody |
Description:
Milli-Mark™ Anti-mOCT4, clone 7F9.2, Alexa Fluor® 488 Conjugate
Trade Name:
Milli-Mark
Specificity:
Antibody recognizes OCT-4 [POU5F1]. N-terminus.
Molecular Weight:
39 kDa Observed
Epitope:
N-terminus
Immunogen:
Recombinant Protein corresponding to the N-terminus of human OCT-4 [POU5F1].
Clone:
7F9.2
Isotype:
IgG1κ
Background Information:
Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis, expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
Species Reactivity:
- Mouse
- Human
Species Reactivity Note:
Proven to work on Mouse. Predicted to react on Human based on 100% sequence homology.
Application Notes:
| Working Solution | Cells / test | Total Reaction Volume |
|---|---|---|
| 5 μl | 5 x 105 Cells / 95 μl PBS | 100 μl |
| 10 μl | 1 x 106 Cells / 90 μl PBS | 100 μl |
Control:
Mouse embryonic stem cells.
Quality Assurance:
Evaluated by flow cytometry using mouse embryonic stem cells.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Storage Conditions:
Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
OCT3
OCT4
OTF3
OTF4
Oct-3
Oct-4
OCT4
OTF3
OTF4
Oct-3
Oct-4
Alternate Names:
Octamer-binding transcription factor 3
POU class 5 homeobox 1
POU domain class 5, transcription factor 1
POU-type homeodomain-containing DNA-binding protein
octamer-binding transcription factor-3
POU class 5 homeobox 1
POU domain class 5, transcription factor 1
POU-type homeodomain-containing DNA-binding protein
octamer-binding transcription factor-3
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Flow Cytometry
UniProt Summary:
FUNCTION: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
SUBUNIT STRUCTURE:Interacts with UBE2I.
SUBCELLULAR LOCATION: Nucleus. Note= Expressed in a diffuse and slightly punctuate pattern.
TISSUE SPECIFICTY: Expressed the totipotent and pluripotent stem cells of the pregastrulation embryo. Also expressed in primordial germ cells and in the female germ line. Absent from adult tissues.
DEVELOPMENTAL STAGE: Down-regulated during differentiation to endoderm and mesoderm. Ref.1
INDUCTION: Repressed by retinoic acid (RA).
PTM: Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
SEQUENCE SIMILARITIES: Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. ...
SUBUNIT STRUCTURE:Interacts with UBE2I.
SUBCELLULAR LOCATION: Nucleus. Note= Expressed in a diffuse and slightly punctuate pattern.
TISSUE SPECIFICTY: Expressed the totipotent and pluripotent stem cells of the pregastrulation embryo. Also expressed in primordial germ cells and in the female germ line. Absent from adult tissues.
DEVELOPMENTAL STAGE: Down-regulated during differentiation to endoderm and mesoderm. Ref.1
INDUCTION: Repressed by retinoic acid (RA).
PTM: Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
SEQUENCE SIMILARITIES: Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. ...
Product Name:
Milli-Mark™ Anti-mOCT4, clone 7F9.2, Alexa Fluor® 488 Conjugate
Concentration:
0.5 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 tests
Format:
AlexaFluor®488
Host:
Mouse