Product Images
Flow Cytometry Analysis:
Representative lot data.
FACS analysis using HeLa cells, stained with anti-RPA2 p34 antibody (green), or Sigma #F6...
Milli-Mark Anti-RPA2 p34 -FITC, clone RPA20 1-46
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | Mouse | FITC | Monoclonal Antibody |
Description:
Milli-Mark Anti-RPA2 p34 -FITC Antibody, clone RPA20 1-46 | FCMAB143F
Promotional Text:
Trade Name:
Milli-Mark
Specificity:
Antibody recognizes RPA2
Molecular Weight:
32 kDa Observed
Epitope:
Unknown
Immunogen:
His-tagged recombinant protein corresponding to human RPA2
Clone:
RPA20, 1-46
Isotype:
IgG1κ
Background Information:
RPA is a heterotrimeric protein complex that binds specifically to single-stranded DNA (ssDNA). It is composed of three subunits, RPA1 (70 kDa), RPA2 (32 kDa), and RPA3 (14 kDa), and plays multiple roles in DNA metabolism. RPA is required for DNA replication initiation, as well as replication elongation. At the onset of DNA replication, RPA is loaded onto chromatin after the binding of Cdc45 to origins. RPA is needed for subsequent loading of DNA polymerase and other replication proteins to initiate DNA replication. After DNA replication begins, RPA moves with replication forks, stabilizing ssDNA and assisting in DNA synthesis. In addition to its replication function, RPA is also known to play essential roles in damage repair and recombination.The 32 kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK. Alternate names for RPA32 include replication protein A 32 kDa subunit, RP-A, RF-A, replication factor-A protein 2, p32, p34, RPA2, REPA2, and RPA32.
Species Reactivity:
Human
Species Reactivity Note:
Tested and passed on Human. Unconjugated antibody part number 04-1481 known to react with Human and Mouse.
Control:
HeLa cells
Quality Assurance:
Evaluated by flow cytometry using HeLa cells.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Storage Conditions:
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
RPA2
REPA2
RPA32
RPA34
REPA2
RPA32
RPA34
Alternate Names:
RF-A protein 2
RP-A p32
RP-A p34
Replication factor A protein 2
replication protein A2 (32kD)
replication protein A2, 32kDa
Replication protein A 32kDa subunit
RF-A, replication factor-A protein 2
p32
p34
RPA2
REPA2
RPA32.
RP-A p32
RP-A p34
Replication factor A protein 2
replication protein A2 (32kD)
replication protein A2, 32kDa
Replication protein A 32kDa subunit
RF-A, replication factor-A protein 2
p32
p34
RPA2
REPA2
RPA32.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Flow Cytometry
UniProt Summary:
FUNCTION: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
SUBUNIT STRUCTURE: Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.
SUBCELLULAR LOCATION: Nucleus. Note: Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
PTM: Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.
SUBUNIT STRUCTURE: Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.
SUBCELLULAR LOCATION: Nucleus. Note: Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
PTM: Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2.
Product Name:
Milli-Mark Anti-RPA2 p34 -FITC, clone RPA20 1-46
Concentration:
0.5 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 tests
Format:
FITC
Host:
Mouse