Product Images
Flow Cytometry Analysis:
Representative lot data.
FACS analysis using HeLa cells, stained with anti-RPA1 p70 antibody (green), or Sigma #F...
Milli-Mark Anti-RPA1 p70 -FITC, clone RPA9, 1-30
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | Mouse | FITC | Monoclonal Antibody |
Description:
Milli-Mark Anti-RPA1 p70 -FITC Antibody, clone RPA9, 1-30 | FCMAB144F
Promotional Text:
Trade Name:
Milli-Mark
Specificity:
This antibody recognizes RPA1
Molecular Weight:
~68kDa Observed
Immunogen:
His-tagged recombinant protein corresponding to human RPA1.
Clone:
RPA9, 1-30
Isotype:
IgG1κ
Background Information:
Replication protein A (RPA) is a multisubunit complex involved in DNA mismatch repair (MMR) in association with MSH2, MLH1 , PCNA, and DNA polymerase-delta. RPA is a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA1 p70 kDa is an ssDNA binding protein shown to be phosphorylated by ATM and ATR in response to DNA damage.RPA also functions in the initiation of DNA replication and DNA unwinding at origins of replication.
Species Reactivity:
Human
Species Reactivity Note:
Tested and passed on Human.
Control:
HeLa Cells
Quality Assurance:
Tested by flow cytometry using HeLa Cells.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Storage Conditions:
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
RPA1
REPA1
RF-A
RP-A
RPA70
REPA1
RF-A
RP-A
RPA70
Alternate Names:
RF-A protein 1
RP-A p70
Replication factor A protein 1
Single-stranded DNA-binding protein
replication protein A 70 kDa DNA-binding subunit
replication protein A1 (70kD)
replication protein A1, 70kDa
replication protein A 70 kDa DNA-binding subunit
REPA1
RPA1
HSSB.
RP-A p70
Replication factor A protein 1
Single-stranded DNA-binding protein
replication protein A 70 kDa DNA-binding subunit
replication protein A1 (70kD)
replication protein A1, 70kDa
replication protein A 70 kDa DNA-binding subunit
REPA1
RPA1
HSSB.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Flow Cytometry
UniProt Summary:
FUNCTION: It participates in a very early step in initiation. RP-A is a single-stranded DNA-binding protein. Absolutely required for simian virus 40 DNA replication in vitro.
SUBUNIT STRUCTURE: Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Interacts with RIP and XPA. Interacts with RPA4.
SUBCELLULAR LOCATION: Nucleus.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SEQUENCE SIMILARITIES: Belongs to the replication factor A protein 1 family.
SUBUNIT STRUCTURE: Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Interacts with RIP and XPA. Interacts with RPA4.
SUBCELLULAR LOCATION: Nucleus.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SEQUENCE SIMILARITIES: Belongs to the replication factor A protein 1 family.
Product Name:
Milli-Mark Anti-RPA1 p70 -FITC, clone RPA9, 1-30
Concentration:
0.5 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 tests
Format:
FITC
Host:
Mouse