Product Images
Flow Cytometry Analysis:
Representative lot data.
Flow Cytometry analysis using HeLa cells, stained with anti-CAF1 p150 antibody (green), ...
Milli-Mark Anti-CAF1 p150 -FITC, clone SS1, 1-3
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | Mouse | FITC | Monoclonal Antibody |
Description:
Milli-Mark Anti-CAF1 p150 -FITC Antibody, clone SS1, 1-3 | FCMAB145F
Promotional Text:
Trade Name:
Milli-Mark
Specificity:
Antibody recognizes CAF-1 p150
Molecular Weight:
150 kDa Calculated
Immunogen:
His-tagged recombinant human protein corresponding to CAF-1 p150.
Clone:
SS1, 1-3
Isotype:
IgG1κ
Background Information:
Chromatin assembly factor (CAF) is a heterotrimeric complex that belongs to the histone chaperone family. It is found in yeast, animals and plants, and facilitates the assembly of chromatin during DNA replication and repair. There are three CAF subunits: p48, p60, and p150. The p150 subunit interacts with heterochromatin protein 1, methyl-binding protein 1 and the Bloom’s syndrome DNA-helicase BLM. Mutations in CAF p150 have resulted in defects in embryo development.
Species Reactivity:
Human
Species Reactivity Note:
Tested and passed on Human.
Control:
HeLa cells
Quality Assurance:
Evaluated by flow cytometry using HeLa cells.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Storage Conditions:
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
CHAF1A
CAF
CAF-1
CAF-Ip150
CAF1
CAF1B
CAF1P150
P150
hp150
CAF
CAF-1
CAF-Ip150
CAF1
CAF1B
CAF1P150
P150
hp150
Alternate Names:
CAF-1 subunit A
CAF-I 150 kDa subunit
Chromatin assembly factor I p150 subunit
chromatin assembly factor 1, subunit A (p150)
chromatin assembly factor I (150 kDa)
CAF-I 150 kDa subunit
Chromatin assembly factor I p150 subunit
chromatin assembly factor 1, subunit A (p150)
chromatin assembly factor I (150 kDa)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Flow Cytometry
Entrez Gene Summary:
Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578])
UniProt Summary:
FUNCTION: Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci By similarity. The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
SUBUNIT STRUCTURE: Homodimer. Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. Part of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. CHAF1A binds directly to PCNA and to CBX1. Binds MBD1. Interacts directly with CBX5 via the PxVxL motif. During DNA replication, it forms a S phase-specific complex that facilitates DNA methylation and histone H3 'Lys-9' methylation during replication-coupled chromatin assembly and is at least composed of the CHAF1A, MBD1 and SETDB1. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B.
SUBCELLULAR LOCATION: Nucleus. Note: DNA replication foci.
DEVELOPMENTAL STAGE: Active complex is found in G1, S and G2 phases.
DOMAIN: Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SEQUENCE SIMILARITIES: Belongs to the CHAF1A family.
SUBUNIT STRUCTURE: Homodimer. Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. Part of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. CHAF1A binds directly to PCNA and to CBX1. Binds MBD1. Interacts directly with CBX5 via the PxVxL motif. During DNA replication, it forms a S phase-specific complex that facilitates DNA methylation and histone H3 'Lys-9' methylation during replication-coupled chromatin assembly and is at least composed of the CHAF1A, MBD1 and SETDB1. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B.
SUBCELLULAR LOCATION: Nucleus. Note: DNA replication foci.
DEVELOPMENTAL STAGE: Active complex is found in G1, S and G2 phases.
DOMAIN: Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SEQUENCE SIMILARITIES: Belongs to the CHAF1A family.
Product Name:
Milli-Mark Anti-CAF1 p150 -FITC, clone SS1, 1-3
Concentration:
0.5 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 tests
Format:
FITC
Host:
Mouse