Product Images
Flow Cytometry Analysis:
Representative lot data.
FACS analysis using HeLa cells, stained with anti-BMI1 antibody (green), or Sigma #F6397...
Milli-Mark Anti-BMI1 -FITC, clone AF27
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | Mouse | FITC | Monoclonal Antibody |
Description:
Milli-Mark Anti-BMI1 -FITC Antibody, clone AF27 | FCMAB149F
Promotional Text:
Trade Name:
Milli-Mark
Specificity:
Antibody recognizes BMI1.
Molecular Weight:
35kDa Observed
Immunogen:
MBP-tagged recombinant protein corresponding to full length human BMI1.
Clone:
AF27
Isotype:
IgG1κ
Background Information:
The BMI1 protein is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors. Several lines of evidence implicate BMI1 in tumorigenesis. BMI1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. BMI1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene, leads to neoplastic transformation. The BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. The transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires BMI1.
Species Reactivity:
Human
Species Reactivity Note:
Tested and passed on Human.
Control:
HeLa cells.
Quality Assurance:
Evaluated by flow cytometry using HeLa cells.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1κ conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Storage Conditions:
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
MGC12685
PCGF4
RNF51
flvi-2/bmi-1
PCGF4
RNF51
flvi-2/bmi-1
Alternate Names:
B lymphoma Mo-MLV insertion region 1 homolog
B lymphoma Mo-MLV insertion region 1 homolog (mouse)
BMI1 polycomb ring finger oncogene
Polycomb group RING finger protein 4
RING finger protein 51
murine leukemia viral (bmi-1) oncogene homolog
oncogene BMI-1
polycomb group protein Bmi1
polycomb group ring finger 4
B lymphoma Mo-MLV insertion region 1 homolog (mouse)
BMI1 polycomb ring finger oncogene
Polycomb group RING finger protein 4
RING finger protein 51
murine leukemia viral (bmi-1) oncogene homolog
oncogene BMI-1
polycomb group protein Bmi1
polycomb group ring finger 4
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Flow Cytometry
UniProt Summary:
FUNCTION: Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
SUBUNIT STRUCTURE: Component of chromatin-associated class II polycomb repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2. Also component of E3 ubiquitin ligase complex or human polycomb repressive complex 1-like at least composed of PHC2, BMI1, RING1 and RNF2. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
PTM: May be polyubiquitinated; which does not lead to proteasomal degradation.
INVOLVEMENT IN DISEASE: Cooperates with the MYC oncogene to produce B-lymphomas.
SEQUENCE SIMILARITIES: Contains 1 RING-type zinc finger
SUBUNIT STRUCTURE: Component of chromatin-associated class II polycomb repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2. Also component of E3 ubiquitin ligase complex or human polycomb repressive complex 1-like at least composed of PHC2, BMI1, RING1 and RNF2. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
PTM: May be polyubiquitinated; which does not lead to proteasomal degradation.
INVOLVEMENT IN DISEASE: Cooperates with the MYC oncogene to produce B-lymphomas.
SEQUENCE SIMILARITIES: Contains 1 RING-type zinc finger
Product Name:
Milli-Mark Anti-BMI1 -FITC, clone AF27
Concentration:
0.5 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 tests
Format:
FITC
Host:
Mouse