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  • : FCMAB171C5
  • : 100 tests
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.
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Flow Cytometry Analysis:
Representative lot data.
FACS analysis of anti-human CD4-PE/Cy5 stained human lymphocytes. Cells expressing CD4 ...

Milli-Mark™ Anti-CD4 -PECy5, clone MT310

Species Reactivity Key Applications Host Format Antibody Type
H FC Mouse Phycoerythrin/Cy5 Monoclonal Antibody
Description:
Milli-Mark™ Anti-CD4 -PECy5 Antibody, clone MT310
Promotional Text:
Trade Name:
Milli-Mark
Specificity:
Antibody recognizes Human CD4 antigen.
Molecular Weight:
55 kDa (Leong, 1999)
Clone:
MT310
Isotype:
IgG1κ
Background Information:
CD4 is a 55 kDa glycoprotein with four immunoglobulin-like external domains, a transmembrane domain and a highly conserved intracellular domain (Leong, 1999). CD4 acts as a coreceptor in MHC class II-restricted antigen-induced T-cell activation. Further, CD4 has a role in the regulation of T-B lymphocyte adhesion in the absence of antigen recognition, and in thymic differentiation (Piatier-Tonneau, 2002). CD4 is expressed on thymocyte subsets, it marks helper/inducer T cells, and it is expressed in 55-65% of mature peripheral T cells. Subpopulations of suppressor or cytotoxic T cells can be identified among CD4-positive T cells. CD4 is also expressed on monocytes/macrophages, Langerhans cells and other dendritic cells. CD4 is not expressed on B cells. Anti-CD4, MT310, was included in the Second International Workshop and Conference on Human Leucocyte Differentiation Antigens, and studies by a number of laboratories confirmed its reactivity with the CD4 antigen (Miedema, 1986).
Species Reactivity:
Human
Species Reactivity Note:
Tested and passed on Human.
Control:
Human Lymphocytes
Quality Assurance:
Evaluated by flow cytometry using human lymphocytes.
Purification Method:
Protein A purfied
Presentation:
Purified Mouse monoclonal IgG1 kappa conjugated to PE-Cy5 in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2
Storage Conditions:
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Alternate Names:
T-cell surface antigen T4/Leu-3
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Flow Cytometry
Product Name:
Milli-Mark™ Anti-CD4 -PECy5, clone MT310
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 tests
Format:
Phycoerythrin/Cy5
Host:
Mouse
Applications:
Detect CD4 using this Milli-Mark Anti-CD4 -PECy5 Antibody, clone MT310 validated for use in FC.

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