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Flow Cytometry Analysis:
Representative lot data.
FC analysis of human leukocytes stained with anti-human CD61-APC antibody. Cells expres...
Milli-Mark™ Anti-CD61-APC, clone Y2/51
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | Mouse | Purified | Monoclonal Antibody |
Description:
Milli-Mark™ Anti-CD61-APC, clone Y2/51
Trade Name:
Milli-Mark
Specificity:
Antibody recognizes Human CD61 antigen.
Molecular Weight:
110 kDa (Sun, 1997)
Immunogen:
Phytohemagglutinin (PHA)-stimulated blood cells
Clone:
Y2/51
Background Information:
CD61 is a selective marker of platelets and platelet precursors (Sun, 1997). CD61 is a 110 KDa single-chain protein and is the common β-subunit of the CD41/CD61 and the CD51/CD61 complexes, both of which are non-covalently associated heterodimers. The complexes require divalent cations for complex integrity and receptor functions (Sun, 1997).
The CD41/CD61 complex appears early in megakaryocyte maturation (Vinci, 1984). The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen and fibronectin (Sun, 1997) and plays a central role in platelet activation and aggregation (Nachman, 1982).
Anti-CD61, Y2/51, was included in the Fourth International Workshop and Conference on Human Leucocyte Differentiation Antigens and studies by a number of laboratories confirmed its reactivity with CD61 (von dem Borne, 1989).
The CD41/CD61 complex appears early in megakaryocyte maturation (Vinci, 1984). The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen and fibronectin (Sun, 1997) and plays a central role in platelet activation and aggregation (Nachman, 1982).
Anti-CD61, Y2/51, was included in the Fourth International Workshop and Conference on Human Leucocyte Differentiation Antigens and studies by a number of laboratories confirmed its reactivity with CD61 (von dem Borne, 1989).
Species Reactivity:
Human
Species Reactivity Note:
Tested and passed on Human.
Control:
Human Leukocytes
Quality Assurance:
Evaluated by flow cytometry using human leukocytes
Purification Method:
Protein A Purified
Presentation:
Purified Mouse monoclonal IgG1 conjugated to APC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
Storage Conditions:
Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Alternate Names:
- Integrin beta-3
- Platelet membrane glycoprotein IIIa
- GPIIIa
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Flow Cytometry
Entrez Gene Summary:
The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
UniProt Summary:
Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Product Name:
Milli-Mark™ Anti-CD61-APC, clone Y2/51
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 tests
Format:
Purified
Host:
Mouse