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Flow Cytometry Analysis:
Representative lot data.
FC analysis of U266 cells stained with anti-human CD138-APC antibody. Cells expressing ...
Milli-Mark™ Anti-CD138-APC, clone MI15
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | Mouse | Purified | Monoclonal Antibody |
Description:
Milli-Mark™ Anti-CD138-APC, clone MI15
Trade Name:
Milli-Mark
Specificity:
Antibody recognizes Human CD138 antigen.
Molecular Weight:
85-92 kDa (Wijdenes, 1997)
Immunogen:
A mixture of U266 and XG-1 human myeloma cell lines
Clone:
MI15
Isotype:
IgG1κ
Background Information:
CD138 is a transmembrane proteoglycan, bearing heparin sulphates and sometimes chondriotin sulfates. It consists of a 30.5 kDa core protein with 5 putative glycosaminoglycans attachment sites. CD138 is a member of the syndecan family. The reported Mr of CD138 ranges from 85,000 to 92,000, presumably depending on the glycosaminoglycan pattern of the molecule (Wijdenes, 1997). Within the hematopoietic system, CD138 is mainly confined to late stages of B-cell differentiation. It is typically expressed at high cellular density in plasma cells (Gattei, 1999). In mature tissues, CD138 is expressed by simple and stratified epithelia, fibroblasts, stratified keratinocytes and endothelial cells (Wijdenes, 1997). Anti-CD138, MI15, was included in the Sixth International Workshop and Conference on Human Leucocytes Differentiation Antigens, and studies by a number of different laboratories confirmed its reactivity with CD138 (Wijdenes, 1997).
Species Reactivity:
Human
Species Reactivity Note:
Tested and passed on Human.
Control:
U266 cells
Quality Assurance:
Evaluated by flow cytometry using U266 cells
Purification Method:
Protein A Purified
Presentation:
Purified Mouse monoclonal IgG1 conjugated to APC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
Storage Conditions:
Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Alternate Names:
- Syndecan-1
- SYND1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Flow Cytometry
Entrez Gene Summary:
The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein.
UniProt Summary:
Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.
Product Name:
Milli-Mark™ Anti-CD138-APC, clone MI15
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 tests
Format:
Purified
Host:
Mouse