Product Images
Flow Cytometry Analysis:
Representative lot data.
FC analysis of anisomycin treated jurkat cells stained with phospho-ATF2-Alexa Fluor® 64...
Milli-Mark™ Anti-phospho-ATF2 (Thr69/71), Alexa Fluor® 647, clone AW65
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | Mouse | AlexaFluor®647 | Monoclonal Antibody |
Description:
Milli-Mark™ Anti-phospho-ATF2 (Thr69/71), Alexa Fluor® 647, clone AW65
Trade Name:
Milli-Mark
Specificity:
Antibody recognizes phospho-ATF2-(Thr69/71) antigen.
Molecular Weight:
55 kDa Calculated
Immunogen:
KLH-conjugated, synthetic peptide corresponding to phospho-threonine at residues 69 and 71 of human ATF2
Modifications:
Phosphorylation
Clone:
AW65
Isotype:
IgG1
Background Information:
Activating transcription factor 2 (ATF2) is a member of the basic region leucine zipper (bZIP) family of proteins. Transcriptionally active ATF2 recognizes and binds to specific ATF/CRE motifs as homo- or heterodimers with other bZIP transcription factors of the ATF/CREB or AP-1 families. ATF2 is regulated by phosphorylation on Thr69/71. Two major kinases known to phosphorylate these sites are the two Stress Activated Protein Kinases (SAPK) Jun and p38 that are mitogen activated protein kinases (MAPKs). Phosphorylation and dimerization are required for the full transcriptional activity of ATF2. Transcriptional regulation by ATF2 has been implicated in mediating many diverse cellular processes such as growth, differentiation, immune and stress responses, inflammatory cytokine signaling, and oncogenic transformation.
Species Reactivity:
Human
Species Reactivity Note:
Tested and passed on Human.
Control:
Jurkat cells
Quality Assurance:
Evaluated by flow cytometry using anisomycin treated jurkat cells
Purification Method:
Protein G Purified
Presentation:
Purified Mouse monoclonal IgG1 conjugated to Alexa Fluor 647 in PBS buffer with 15mg/mL BSA, Sodium azide at 0.1%.
Storage Conditions:
Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
ATF2, CREB2, CREBP1
Alternate Names:
- Cyclic AMP-dependent transcription factor ATF-2
- cAMP-dependent transcription factor ATF-2
- Activating transcription factor 2
- cAMP response element-binding protein CRE-BP1
- HB16
- cAMP-responsive element-binding protein 2
- CREB-2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Flow Cytometry
Entrez Gene Summary:
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined
UniProt Summary:
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'- GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.
Product Name:
Milli-Mark™ Anti-phospho-ATF2 (Thr69/71), Alexa Fluor® 647, clone AW65
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 tests
Format:
AlexaFluor®647
Host:
Mouse