Product Images
Flow Cytometry Analysis:
Representative lot data.
FC analysis of HeLa cells stained with Anti-Ubiquitin, Lys63-Specific, Alexa Fluor® 647 ...
Milli-Mark™ Anti-Ubiquitin, Lys63-Specific-Alexa Fluor®647, clone Apu3
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | Rabbit | AlexaFluor®647 | Polyclonal Antibody |
Description:
Milli-Mark™ Anti-Ubiquitin, Lys63-Specific-Alexa Fluor®647, clone Apu3
Trade Name:
Milli-Mark
Specificity:
Recognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin.
Molecular Weight:
Molecular weight varies.
Epitope:
Lys63 ubiquitination site
Immunogen:
Immunogen used was di-ubiquitin linked at Lys63.
Clone:
Apu3
Isotype:
IgG
Background Information:
Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.
Species Reactivity:
Human
Species Reactivity Note:
Tested and passed on Human.
Control:
HeLa cells
Quality Assurance:
Evaluated by flow cytometry using HeLa cells
Purification Method:
Protein A Purified
Presentation:
Purified recombinant rabbit monoclonal IgG1 conjugated to Alexa Fluor 647 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Storage Conditions:
Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.
UniProt Number:
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Flow Cytometry
UniProt Summary:
FUNCTION: Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ref.21
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly.
MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
SIMILARITY: Belongs to the ubiquitin family.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly.
MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
SIMILARITY: Belongs to the ubiquitin family.
Protein/Isoform Description:
Ubiquitin is small protein that is covalently attached to particular lysine residues on other proteins, and a poly-ubiquitin chain develops on those proteins to mark them for degradation. Regulated degradation is critical for correct cell cycle progression, activation of NFκB, and p53 turnover. In addition, certain histones appear to be regulated by addition of a single ubiquitin molecule.
Product Name:
Milli-Mark™ Anti-Ubiquitin, Lys63-Specific-Alexa Fluor®647, clone Apu3
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 tests
Format:
AlexaFluor®647
Host:
Rabbit