Product Images
Flow Cytometry Analysis:
Representative lot data.
FC analysis of U251 cells stained with anti-MAP-2-PE (yellow) or isotype control (grey).
Milli-Mark™ Anti-MAP2-PE, clone AP20
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | Mouse | Phycoerythrin | Monoclonal Antibody |
Description:
Milli-Mark™ Anti-MAP2-PE, clone AP20
Trade Name:
Milli-Mark
Specificity:
Antibody recognizes MAP-2.
Molecular Weight:
199.5 kDa Calculated
Immunogen:
Recombinant Bovine brain microtubule protein.
Clone:
AP20
Isotype:
IgG1
Background Information:
MAP-2 (microtubule associated protein-2) is one of several high molecular weight proteins that play an important role in brain neuron microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles. MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2. MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds. (Caceres, 1986; Dotti, 1987).
Species Reactivity:
Human
Species Reactivity Note:
Tested and passed on Human. Predicted to react on Bovine based on immunogen designed.
Control:
U251 cells
Quality Assurance:
Evaluated by flow cytometry using U251 cells
Purification Method:
Protein A Purified
Presentation:
Purified mouse monoclonal IgG1 conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Storage Conditions:
Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
MAP2
Alternate Names:
Microtubule-associated protein 2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Flow Cytometry
Entrez Gene Summary:
This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described.
UniProt Summary:
FUNCTION: SwissProt: P11137 # The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
SIZE: 1827 amino acids; 199539 Da
PTM: MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: P11137 ## Contains 3 Tau/MAP repeats.
SIZE: 1827 amino acids; 199539 Da
PTM: MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: P11137 ## Contains 3 Tau/MAP repeats.
Product Name:
Milli-Mark™ Anti-MAP2-PE, clone AP20
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 tests
Format:
Phycoerythrin
Host:
Mouse