Product Images
Flow Cytometry Analysis:
Representative lot data.
FC analysis of A431 cells stained with Anti-β-Tubulin, FITC conjugate. Cells were stain...
Milli-Mark™ Anti-β-Tubulin-FITC, clone AA2
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, B | FC | Mouse | FITC | Monoclonal Antibody |
Description:
Milli-Mark™ Anti-β-Tubulin-FITC, clone AA2
Trade Name:
Milli-Mark
Specificity:
Antibody recognizes Human beta tubulin
Molecular Weight:
50 kDa Calculated
Immunogen:
Purified bovine brain tubulin. The epitope recognized by this antibody has been mapped to amino acids 412-430.
Clone:
AA2
Isotype:
IgG1
Background Information:
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each about 55 kDa in size. Beta-tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified . Beta tubulin influences the stability of the heterdimer, determined by whether the molecule of GTP bound to the beta tubulin subunit hydrolyzes to GDP along the microtubule protofilament. Beta tubulin has also been a target for various antitubulin agents used in the treatment of cancer. Studies have concluded that beta tubulin mutatons can contribute to drug resistance and may play a role in resistance to chemotherapy.
Species Reactivity:
- Human
- Mouse
- Rat
- Bovine
Species Reactivity Note:
Tested and passed on Human. Predicted to react on bovine based on immunogen design.
Control:
A431 cells
Quality Assurance:
Evaluated by flow cytometry using A431 cells
Purification Method:
Protein G Purified
Presentation:
Purified mouse monoclonal IgG1 conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA
Storage Conditions:
Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- M40
- MGC117247
- MGC16435
- OK/SW-cl.56
- OTTHUMP00000029069
- TUBB1
- TUBB5
- Tubb5
Alternate Names:
- Tubulin beta-5 chain
- beta 5-tubulin
- beta Ib tubulin
- beta-4 tubulin
- tubulin beta polypeptide
- tubulin beta-1 chain
- tubulin, beta
- tubulin, beta polypeptide
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Flow Cytometry
UniProt Summary:
FUNCTION: SwissProt: P07437 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
SIZE: 444 amino acids; 49671 Da
SUBUNIT: Dimer of alpha and beta chains.
TISSUE SPECIFICITY: Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
DOMAIN: SwissProt: P07437 The highly acidic C-terminal region may bind cations such as calcium.r />SIMILARITY: Belongs to the tubulin family.
SIZE: 444 amino acids; 49671 Da
SUBUNIT: Dimer of alpha and beta chains.
TISSUE SPECIFICITY: Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
DOMAIN: SwissProt: P07437 The highly acidic C-terminal region may bind cations such as calcium.r />SIMILARITY: Belongs to the tubulin family.
Product Name:
Milli-Mark™ Anti-β-Tubulin-FITC, clone AA2
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 tests
Format:
FITC
Host:
Mouse