Product Images
Flow Cytometry Analysis:
Representative lot data.
FC analysis using untreated HeLa cells stained with Anti-RNA Polymerase II FITC antibody...
Milli-Mark™ Anti-RNA Poly II-FITC, clone CTD4H8
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | Mouse | FITC | Monoclonal Antibody |
Description:
Milli-Mark™ Anti-RNA Poly II-FITC, clone CTD4H8
Trade Name:
Milli-Mark
Specificity:
Antibody recognizes human RNA Polymerase II.
Molecular Weight:
217 kDa Calculated
Epitope:
carboxyl-terminal domain
Immunogen:
Peptide corresponding to the carboxyl-terminal domain of RNA polymerase II.
Clone:
CTD4H8
Isotype:
IgG1κ
Background Information:
RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors.
Species Reactivity:
Human
Species Reactivity Note:
Tested and passed on Human. Unconjugated antibody (Cat. No. 05-623) is known to react on Rat, Mouse, Yeast (S. cerevisiae).
Control:
HeLa Cells
Quality Assurance:
Evaluated by flow cytometry using HeLa cells.
Purification Method:
Protein G Purified
Presentation:
Purified mouse monoclonal IgG1κ conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Storage Conditions:
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Flow Cytometry
Entrez Gene Summary:
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA ineukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential forpolymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq]
Protein/Isoform Description:
The large subunit of RNA polymerase II has a large C-terminal domain consisting of 52 exact repeats of the sequence YSPTSPS. Phosphorylation of that domain is regulatory of pol II activity, and therefore of discretionary gene expression. Phosphorylation is very high at initiation of transcription, and in particular Ser 5 of the repeat when phosphorylated stimulates the mRNA capping apparatus (guanylyl transferase) by enhancing its affinity for GTP. Ser 5 is phosphorylated best following mitogen stimulation, is sensitive to MEK inhibitors, and makes for a perfect MAP kinase site which is hit strongly in vitro.
Product Name:
Milli-Mark™ Anti-RNA Poly II-FITC, clone CTD4H8
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 tests
Format:
FITC
Host:
Mouse