Granulocyte-Macrophage Colony-Stimulating Factor, recombinant human

Granulocyte-Macrophage Colony-Stimulating Factor, recombinant human

Chemicon (Millipore)

eGranulocyte Macrophage Colony Stimulating Factor (GM-CSF) is a potent species-specific stimulator of bone marrow cells. GM-CSF stimulates precursor cells of granulocytes, macrophages and eosinophils. Human GM-CSF is a 14 kDa protein containing 123 amino acids residues.

GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines.

Cell Culture

For most in vitro applications, human GM-CSF exerts its biological activity in the concentration range of 0.05 to 0.5 ng/mL. Responding cells are (partial list): Certain BM precursor cells.

Optimal working dilutions must be determined by end user.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

GM-CSF

Cytokines & Growth Factors

• Maintenance & Expansion
• Differentiation
• General Cell Culture

Lyophilized powder with no additives

Hematopoietic Stem Cells

Greater than 98% by SDS-PAGE analysis.

Human GM-CSF is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is <= 0.5 ng/mL, corresponding to a specific activity of >= 2 x 10e6 units/mg.

The lyophilized powder, though stable at room temperature, is best stored desiccated below 0°C. Reconstitute with sterile water to a concentration of 0.1 mg/mL. This solution can be diluted into other buffered solutions. After reconstitution maintain at -20°C in undiluted aliquots for up to six months.

E.coli

• IL3
• MGC79399
• MCGF
• MULTI-CSF
• MGC79398
• IL-3

Growth Factor

Cytokines & Growth Factors

Human