Insulin-like Growth Factor-II, recombinant human
Description:
Insulin-like Growth Factor-II, recombinant human
Trade Name:
Chemicon (Millipore)
Product Overview:
Insulin Like Growth Factor-II (IGF-II) is a polypeptide growth factor which stimulates the proliferation of a wide range of cell types. Human IGF-II is a 7.5 kDa protein containing 67 amino acid residues.
Key Applications:
Cell Culture
Application Notes:
For most in vitro applications, IGF-II exerts its biological activity in the concentration range of 0.5 to 25.0 ng/mL. Responding cells are (partial list): Bone, muscle cells.
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Alternate Names:
IGF-II
Cell Culture Reagent Type:
Cytokines & Growth Factors
Descriptive Text:
Stem Cell Workflow Stage:
- Maintenance & Expansion
- Differentiation
- General Cell Culture
Presentation:
Lyophilized powder with no additives
Stem Cell Type:
Mesenchymal Stem Cells
Purity:
Greater than 98% by SDS-PAGE and HPLC analysis
Specific Activity:
Chemicon's Human IGF-II is fully biologically active when compared to standards. The ED50 as determined by a cell proliferation assay using FDC-P1 cells is <= 2.0 ng/mL, corresponding to a specific activity of >= 5 x 105 units/mg.
Storage Conditions:
The lyophilized IGF-II, though stable at room temperature, is best stored at -20°C. After a quick spin, reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into other buffered solutions. Reconstituted IGF-II should be stored in undiluted aliquots at -20°C for up to six months. Avoid repeated freeze/thaw cycles.
Source:
E.coli
Gene Symbol:
- IGF2
- FLJ44734
- pp9974
- ORFNames=PP1446
- C11orf43
- IGF-II
- INSIGF
- Somatomedin-A
- FLJ22066
Protein Type:
Growth Factor
Protein or Enzyme Type:
Cytokines & Growth Factors
Species:
Human