Ordering Information

Catalogue Number : GF020
Qty/Pk : 20 µg

RANTES, recombinant human

Description:

Trade Name:

Chemicon (Millipore)

Qty/Pk:

20 µg

Product Overview:

RANTES is a protein which has been shown to be a chemoattractant for peripheral blood monocytes. It appears to selectively attract T-cells of the CD4+/CD45RO+ phenotype in vitro. Human RANTES is a 7.8 kDa protein containing 68 amino acid residues.

Key Applications:

Cell Culture

Application Notes:

Concentration range for most in vitro applications is 1.0-10 ng/mL. Responding cells are (partial list): Monocytes.

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Entrez Gene Summary:

This gene is one of several CC cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor CCR5 and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor.

UniProt Summary:

FUNCTION: SwissProt: P13501 # Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T- cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
SIZE: 91 amino acids; 9990 Da
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: T-cell and macrophage specific.
PTM: N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells. & The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
SIMILARITY: SwissProt: P13501 ## Belongs to the intercrine beta (chemokine CC) family.

Species:

Human

Brand Family:

Chemicon

Source:

E.coli

Presentation:

Lyophilized protein with no additives.

UniProt Number:

P13501

Specific Activity:

The biological activity of Chemicon's human RANTES was determined by a chemotaxis assay using human blood monocytes. Significant chemotactic activity was obtained at a concentration of 1.0-10 ng/mL.

Storage Conditions:

The lyophilized powder, though stable at room temperature, is best stored desiccated below 0°C. Reconstitute in water to a concentration of 100 mg/mL. This solution can be diluted into water or other buffered solutions. Reconstituted RANTES should be stored in working aliquots at -20°C for up to 6 months.

Cell Culture Reagent Type:

Cytokines & Growth Factors

Protein/Isoform Description:

RANTES, also known as CCL5, is chemotactic for T-cells, eosinophils and basophils. Its synthesis is induced by TNFα and IL-1α.

Gene Symbol: