Ordering Information
- : GF074
- : 10 µg
Stromal Cell-Derived Factor-1β, recombinant human
Description:
Stromal Cell-Derived Factor-1β, recombinant human
Trade Name:
Chemicon (Millipore)
Qty/Pk:
10 µg
Product Overview:
Stromal Cell-Derived Factor-1beta (SDF1beta) is a recently discovered protein belonging to the alpha-chemokine (C-X-C) family of cytokines. GF074 is an 8.5 kDa protein containing 72 amino acid residues.
Key Applications:
Cell Culture
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
For background information on chemokines, see CXCL11 (SCYB11; MIM 604852). Stromal cell-derived factors 1-alpha and 1-beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF (see MIM 191160), or IL1 (see MIM 147760). The intercrines are characterized by the presence of 4 conserved cysteines which form 2 disulfide bonds. They can be classified into 2 subfamilies. In the CC subfamily, which includes beta chemokine, the cysteine residues are adjacent to each other. In the CXC subfamily, which includes alpha chemokine, they are separated by an intervening amino acid. The SDF1 proteins belong to the latter group.[supplied by OMIM]
UniProt Summary:
FUNCTION: SwissProt: P48061 # Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites.
SIZE: 93 amino acids; 10666 Da
SUBCELLULAR LOCATION: Secreted.
PTM: Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processsed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
SIMILARITY: SwissProt: P48061 ## Belongs to the intercrine alpha (chemokine CxC) family.
SIZE: 93 amino acids; 10666 Da
SUBCELLULAR LOCATION: Secreted.
PTM: Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processsed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
SIMILARITY: SwissProt: P48061 ## Belongs to the intercrine alpha (chemokine CxC) family.
Species:
Human
Stem Cell Type:
Hematopoietic Stem Cells
Stem Cell Workflow Stage:
- Maintenance & Expansion
- Differentiation
- General Cell Culture
Brand Family:
Chemicon
Source:
E.coli
Presentation:
Lyophilized protein with no additives.
UniProt Number:
Specific Activity:
Determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentration range of 20.0-80.0 ng/mL.
Storage Conditions:
The lyophilized powder, though stable at room temperature, is best stored at -20°C. Reconstitute in water to a concentration of 0.1-1 mg/mL. This solution can then be diluted into other aqueous buffers and stored at 4ºC for 1 week or at -20ºC for future use. Reconstituted SDF1beta should be stored in undiluted aliquots at -20°C for up to 6 months.
Cell Culture Reagent Type:
Cytokines & Growth Factors
Gene Symbol:
- CXCL12
- SDF1A
- TLSF-b
- TPAR1
- SDF-1b
- TLSF
- SDF-1
- SDF1
- PBSF
- TLSF-a
- SDF1B
- SCYB12
- hIRH
- SDF-1a
Protein or Enzyme Type:
Cytokines & Growth Factors
Product Name:
Stromal Cell-Derived Factor-1β, recombinant human
Entrez Gene Number:
Alternate Names:
SDF-1beta
Purity:
Greater than 98% by SDS-PAGE and HPLC analysis