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Ordering Information

  • : GLP1T-36HK
  • : 125 tubes

Glucagon Like Peptide-1 (Total) RIA

Description:
Glucagon Like Peptide-1 (Total) RIA
Qty/Pk:
125 tubes
Background Information:
GLP-1 is secreted from gut endocrine cells in response to nutrient ingestion and plays multiple roles in metabolic homeostasis.
Applications:
This radioimmunoassay (RIA) measures & quantifies Glucagon Like Peptide-1 for use in Metabolism & Endocrine research.
Key Applications:
Radioimmunoassay
Application Notes:
Two-day disequilibrium assay at 4°C. Sample volume = 300 μL plasma (extraction required)
Species Reactivity:
Mammals
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.
UniProt Summary:
FUNCTION: SwissProt: P01275 # Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
SIZE: 180 amino acids; 20909 Da
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.
PTM: Proglucagon is post-translationally processed in a tissue- specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
SIMILARITY: SwissProt: P01275 ## Belongs to the glucagon family.
MISCELLANEOUS: In the glucagon antagonist, His-53 and Phe-58 are missing. This antagonist has been successfully utilized to reduce glucose concentration in vivo."
Label:
125I-GLP-1
Gene Symbol:
  • GCG
  • GLP1
  • glucagon
  • GRPP
  • GLP2
Cross Reactivity:
GLP-1 is conserved in almost all mammals. This kit may be used to measure GLP-1 in these samples (e.g. rat, mouse, etc.)
Product Name:
Glucagon Like Peptide-1 (Total) RIA
Standard Curve Range:
10–1000 pM
Analytes Available:
Glucagon Like Peptide-1
UniProt Number:
Precision, %:
  • Inter-assay: 10–23
  • Intra-assay: 22–38
Accuracy:
79–119%
Detection Methods:
  • Radioactive
  • 125I
Performance:
  • ED80 = 14 ± 3 pM
  • ED50 = 53 ± 6 pM
  • ED20 = 224 ± 20 pM
Entrez Gene Number:

Product Resources

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