Northern and Southern Blotting, and Colony and Plaque Lifts
Millipore offers a choice of transfer membranes for nucleic acid applications. For superior results in Southern blotting, northern blotting, and colony/plaque lifts, positively charged Immobilon-Ny+ Transfer Membrane is recommended. Immobilon-NC nitrocellulose transfer membranes, the original matrices used in key Southern and colony plaque protocols, are also available as a less sensitive and more economical alternative.
Biologically inert mixtures of cellulose acetate and cellulose nitrate have made MF-Millipore membrane filters one of the most widely used membranes in analytical and research applications. Triton surfactant-free MF-Millipore filters contain minimum amounts of wetting agent and have a lower water extractable content than standard MF-Millipore filters.