BrdU Assay
(Mouse primary antibody)
Description:
BrdU Assay (Mouse primary antibody)
Product Overview:
Millipore’s HCS201 BrdU HCS assay provides a complete solution for for the in vitro quantitative detection of newly synthesized DNA and quantitation of actively proliferating cells. The reagents in the kit have been specifically optimized for HCS applications.
Background Information:
Cellular processes that influence proliferation are particularly attractive as therapeutic targets, and accordingly, detection of BrdU incorporation has found widespread application in High Content Screening assays. These studies have used HCS detection and analysis of BrdU incorporation for target validation, evaluation of genes identified as potential therapeutic or diagnostic targets, and for cell cycle inhibitor profiling, confirming the usefulness of this technique as a tool for drug discovery.
Key Applications:
in vitro Toxicology Assays
Application Notes:
Cell Cycle
Species Reactivity:
Mouse
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Therapeutic Areas:
Oncology
Antigen:
BrdU
Host Cells:
HeLa cell line
Materials Required but Not Delivered:
1. Sterile, tissue culture-treated black/clear bottom microplates suitable for High-Content Imaging.
2. Cell-type for assay, e.g., HeLa (human cervical adenocarcinoma, ATCC #CCL-2) or A549 (human lung carcinoma, ATCC #CCL-185).
3. Tissue culture instruments/supplies (including 37ºC incubator, growth media, flasks/plates, detachment buffer, etc.) for cell type of interest.
4. HCS imaging/analysis system, e.g., GE Healthcare IN Cell Analyzer 1000 with Investigator software. Imaging system must be equipped with beam-splitters and filters capable of reading emission spectra in the blue and red ranges.
2. Cell-type for assay, e.g., HeLa (human cervical adenocarcinoma, ATCC #CCL-2) or A549 (human lung carcinoma, ATCC #CCL-185).
3. Tissue culture instruments/supplies (including 37ºC incubator, growth media, flasks/plates, detachment buffer, etc.) for cell type of interest.
4. HCS imaging/analysis system, e.g., GE Healthcare IN Cell Analyzer 1000 with Investigator software. Imaging system must be equipped with beam-splitters and filters capable of reading emission spectra in the blue and red ranges.
Kit Type:
High-Content Screening
Components:
- 1. Mouse Anti-BrdU HCS Primary Antibody, 5X
- 2. HCS Secondary Antibody (donkey anti-mouse IgG, Cy3 conjugate), 200X
- 3. Hoechst HCS Nuclear Stain, 200X
- 4. BrdU, 250X
- 5. HCS Fixation Solution with Phenol Red, 1X
- 6. HCS Immunofluorescence Buffer, 1X
- 7. HCS Wash Buffer, 1X
- 8. Aphidicolin (G1/S Arrest Control Compound), 250X
- 9. Nocodazole (G2/M Arrest Control Compound), 250X
- 10. DMSO for Compound Serial Dilution
- 11. Compound Dilution Buffer
- 12. Plate Sealers
Configuration:
Sufficient reagents for 5 X 96 well plates
Detection Methods:
Fluorescent
Kit or Assay Type:
High-Content Screening
Storage Conditions:
When stored under the conditions indicated on the labels, the kit components are stable up to the expiration date. HCS Fixation Solution, HCS Immunofluorescence Buffer, HCS Wash Buffer, DMSO, Compound Dilution Buffer, and Plate Sealers should be stored at 2-8ºC. HCS Primary Antibody, HCS Secondary Antibody, Hoechst HCS Nuclear Stain, BrdU, Aphidicolin and Nocodazole should be stored at -20ºC, avoiding repeated freeze/thaw cycles. (Note: If kit is expected to be used for multiple experiments, rather than a single use, thaw antibodies, nuclear stain and control compounds and dispense into appropriately sized aliquots. Store aliquots at -20ºC.)
Discard any remaining reagents after the expiration date.
Discard any remaining reagents after the expiration date.
Host:
Mouse