Informazioni per l’ordine
Ki-67 Assay
(Rabbit primary antibody)
Description:
Ki-67 Assay
(Rabbit primary antibody)
(Rabbit primary antibody)
Product Overview:
Millipore's HCS205 assay provides a complete solution for identifying and quantifying Ki-67 expression in cellular imaging applications. The reagents in the kit have been specifically optimized for HCS applications.
Background Information:
The Ki-67 antigen (Ki-67) is a classic marker of cellular proliferation and has found widespread application in diagnostic, research and drug discovery applications. The Ki-67 antigen was originally defined by the monoclonal antibody Ki-67, the name being derived from the city of origin (Kiel) and the number of the original clone in the 96-well plate. Ki-67 antigen is preferentially expressed during late G1, S, G2 and M phase of the cell cycle, while resting, non-cycling cells (G0 phase) lack Ki-67 expression. Thus, Ki-67 is commonly used as a proliferation marker.
Key Applications:
- in vitro Toxicology Assays
- Immunocytochemistry
- High Content Analysis
Application Notes:
Cell Cycle, Drug screening
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Kit or Assay Type:
High-Content Screening
Configuration:
Sufficient reagents for 5 X 96 well plates
Components:
- 1. Rabbit Anti-Ki-67 HCS Primary Antibody, 100X
- 2. HCS Secondary Antibody (donkey anti-rabbit IgG, Cy3 conjugate), 200X
- 3. Hoechst HCS Nuclear Stain, 200X
- 4. HCS Fixation Solution with Phenol Red, 2X
- 5. HCS Immunofluorescence Buffer, 1X
- 6. HCS Wash Buffer, 1X
- 7. Camptothecin (G1/S Arrest Control Compound)
- 8. Vinblastine Sulfate (G2/M Arrest Control Compound), 250X
- 9. DMSO for Compound Serial Dilution
- 10. Compound Dilution Buffer
- 11. Plate Sealers
Storage Conditions:
Store kit components under the conditions indicated on the labels. Discard any remaining reagents after 6 months.
Detection Methods:
Fluorescent
Materials Required but Not Delivered:
1. Sterile, tissue culture-treated black/clear bottom microplates suitable for High-Content Imaging.
2. Cell-type for assay, e.g., HeLa (human cervical adenocarcinoma, ATCC #CCL-2) or A549 (human lung carcinoma, ATCC #CCL-185).
3. Tissue culture instruments/supplies (including 37ºC incubator, growth media, flasks/plates, detachment buffer, etc.) for cell type of interest.
4. HCS imaging/analysis system, e.g., GE Healthcare IN Cell Analyzer 1000 with Investigator software. Imaging system must be equipped with beam-splitters and filters capable of reading emission spectra in the blue and red ranges.
2. Cell-type for assay, e.g., HeLa (human cervical adenocarcinoma, ATCC #CCL-2) or A549 (human lung carcinoma, ATCC #CCL-185).
3. Tissue culture instruments/supplies (including 37ºC incubator, growth media, flasks/plates, detachment buffer, etc.) for cell type of interest.
4. HCS imaging/analysis system, e.g., GE Healthcare IN Cell Analyzer 1000 with Investigator software. Imaging system must be equipped with beam-splitters and filters capable of reading emission spectra in the blue and red ranges.