订购信息
Neurotoxicity and Neurite Outgrowth Assay for High Content Screening
Recommended Replacement for: HCS200Description:
Neurotoxicity and Neurite Outgrowth Assay
for High Content Screening
for High Content Screening
Product Overview:
Millipore’s HCS220 Neurotoxicity and Neurite Outgrowth Assay is a High Content Screening kit comprised of high-quality, validated, target-specific detection reagents for profiling neurotoxicity, neurite outgrowth and neuronal morphology in a wide variety of mammalian cell types. The highly-validated reagents provided with this kit allow the user to standardize their assays, minimize assay-to-assay variability, and to reproducibly generate images with a high signal-to-background ratio.
Background Information:
During development, neurons and neuronal like cells extend numerous processes that differentiate into dendrites and axons. These processes, known as neurites, are critical for communication between neurons. The characterization of neurite formation, maturation and collapse/resorption is an area of intense interest, since these cellular processes are essential for interconnection of neuronal cell bodies. Neurite formation and neuronal regeneration hold particular promise for development of therapies for neurodegenerative conditions such as Alzheimer's and Parkinson's diseases, neuronal regeneration following spinal cord or brain injury, stroke, and diabetic neuropathy . Therefore, major efforts in central nervous system drug discovery research are focused on the identification of compounds that affect neurite outgrowth.
The current drug development process is slow, and results in a fail rate exceeding 90%. The development of High Content Screening (HCS) technology represents a major step towards improving the drug discovery and development process. High Content Screening enables the evaluation of multiple biochemical and morphological parameters in cellular systems. By combining the automated imaging of cells in microtiter plates with high-quality detection reagents and powerful image analysis algorithms, scientists can now acquire deeper knowledge of multiple morphological or biochemical pathways at the single-cell level at an early stage in the development of new drugs. HCS platforms such as GE's IN Cell Analyzer 1000, when coupled with advanced reagents and informatics tools, can be used to deliver detailed profiles of cellular systemic responses, rather than merely observing single cellular events.
The use of HCS for measurement of neurite outgrowth has become a valuable tool for neuroscience research and drug discovery. Notably, HCS has been demonstrated to be an effective method for determination of inducers and inhibitors of neurite outgrowth. HCS provides the opportunity to perform high-throughput, non-subjective, quantitative neurite outgrowth assays, and enables morphological screening of multiple parameters in individual cells, such as neurite count, total neurite length, mean neurite length and branch point count. Additionally, HCS analysis of neuronal morphology provides a more effective method for quantification of non-lethal forms of neuronal cell injury than traditional cytotoxicity assays. These advantages of HCS neurite outgrowth assays offer the opportunity for greatly improved productivity in neuroscience research and drug discovery. A large number of cell types have been successfully utilized for HCS neurite outgrowth analysis. These include immortalized/stable cell lines such as PC12, SY5Y and N2A; primary hippocampal, cortical and dorsal root ganglia (DRG) neurons; and neuronal embryonic stem cells.
Successful HCS assays rely on high-quality detection reagents. With the commercial availability of tens of thousands of immunoreagents and fluorescent probes, large numbers of fixed end-point HCS assays are possible. However, immunoreagents for HCS assays carry special requirements. Strong antigen affinity is required, minimal non-specific binding must be observed, and the signal to background ratio must be sufficient to ensure an adequate screening window.
Millipore's HCS200 Neurite Outgrowth Assay is a High Content Screening kit comprised of high-quality, validated, target-specific detection reagents for profiling neurite outgrowth and neuronal morphology in a wide variety of mammalian cell types.
The current drug development process is slow, and results in a fail rate exceeding 90%. The development of High Content Screening (HCS) technology represents a major step towards improving the drug discovery and development process. High Content Screening enables the evaluation of multiple biochemical and morphological parameters in cellular systems. By combining the automated imaging of cells in microtiter plates with high-quality detection reagents and powerful image analysis algorithms, scientists can now acquire deeper knowledge of multiple morphological or biochemical pathways at the single-cell level at an early stage in the development of new drugs. HCS platforms such as GE's IN Cell Analyzer 1000, when coupled with advanced reagents and informatics tools, can be used to deliver detailed profiles of cellular systemic responses, rather than merely observing single cellular events.
The use of HCS for measurement of neurite outgrowth has become a valuable tool for neuroscience research and drug discovery. Notably, HCS has been demonstrated to be an effective method for determination of inducers and inhibitors of neurite outgrowth. HCS provides the opportunity to perform high-throughput, non-subjective, quantitative neurite outgrowth assays, and enables morphological screening of multiple parameters in individual cells, such as neurite count, total neurite length, mean neurite length and branch point count. Additionally, HCS analysis of neuronal morphology provides a more effective method for quantification of non-lethal forms of neuronal cell injury than traditional cytotoxicity assays. These advantages of HCS neurite outgrowth assays offer the opportunity for greatly improved productivity in neuroscience research and drug discovery. A large number of cell types have been successfully utilized for HCS neurite outgrowth analysis. These include immortalized/stable cell lines such as PC12, SY5Y and N2A; primary hippocampal, cortical and dorsal root ganglia (DRG) neurons; and neuronal embryonic stem cells.
Successful HCS assays rely on high-quality detection reagents. With the commercial availability of tens of thousands of immunoreagents and fluorescent probes, large numbers of fixed end-point HCS assays are possible. However, immunoreagents for HCS assays carry special requirements. Strong antigen affinity is required, minimal non-specific binding must be observed, and the signal to background ratio must be sufficient to ensure an adequate screening window.
Millipore's HCS200 Neurite Outgrowth Assay is a High Content Screening kit comprised of high-quality, validated, target-specific detection reagents for profiling neurite outgrowth and neuronal morphology in a wide variety of mammalian cell types.
Key Applications:
- Neuroscience
- Neurotoxicity
- High Content Analysis
- Immunocytochemistry
Application Notes:
High Content imaging and analysis of neurite outgrowth and neuronal cell
morphology.
morphology.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Kit or Assay Type:
High-Content Screening
Configuration:
Sufficient reagents for 5 X 96 well plates
Components:
- 1. Mouse Beta III Tubulin HCS Primary Antibody, 200x
- 2. HCS Secondary Antibody - FITC gt anti ms IgG, 1 mg/mL 200x
- 3. Hoechst HCS Nuclear Stain
- 4. HCS Fixation Solution, 1x
- 5. Immunofluorescence Buffer for HCS, 1x
- 6. HCS Wash Buffer, 1x
- 7. Plate Sealers
Storage Conditions:
Store kit components under the conditions indicated on the labels. Discard any remaining reagents after 6 months.
Detection Methods:
Fluorescent
Replaces:
HCS200