Informazioni per l’ordine
Immagini del prodotto
MnSOD dose responses of A549, HeLa and HepG2 cells to toxic stresses.
A549 or HeLa cells were plated at 18,000 cells/cm2 (37,000 cells/cm2 for H...
HCS232 Rabbit Anti-MnSOD Assay reagent stability.
HeLa cells were seeded at 18,000 cells/cm2 on 96-well plates in growth media and cultured for 2...
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Manganese Superoxide Dismutase (MnSOD) Assay
Description:
Manganese Superoxide Dismutase (MnSOD) Assay
Product Overview:
Millipore’s HCS232 assay provides a complete solution for identifying and quantifying manganese superoxide dismutase (MnSOD) in cellular imaging studies. The reagents in the kit have been specifically optimized for HCS applications.The assay is designed to enable visualization and quantitative detection of MnSOD, facilitating the characterization of the cellular oxidative stress response, and screening of compounds that may induce, inhibit or repair cellular injury. The nuclear dye (Hoechst 33342) may be used for measurements of cell number, DNA content and nuclear size. Additionally, the assay can be multiplexed with other probes, e.g., for apoptotic pathway studies, oncology drug efficacy trials or in vitro toxicology applications.
Background Information:
Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3). The manganese containing 80 kDa tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain.
Key Applications:
- Immunocytochemistry
- Immunofluorescence
- in vitro Toxicology Assays
- High Content Analysis
Application Notes:
Oxidative Stress
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Kit or Assay Type:
- High-Content Screening
- High-Content Analysis
Configuration:
Sufficient reagents for 5 X 96 well plates
Components:
- 1. Rabbit Anti-Manganese Superoxide Dismutase (MnSOD) HCS Primary Antibody, 100X
- 2. HCS Secondary Antibody (donkey anti-rabbit IgG, Cy3 conjugate), 200X
- 3. Hoechst HCS Nuclear Stain, 200X
- 4. HCS Fixation Solution with Phenol Red, 2X
- 5. HCS Immunofluorescence Buffer, 1X
- 6. HCS Wash Buffer, 1X
- 7. Etoposide (S/G2 Arrest Control Compound), 250X
- 8. Camptothecin (Control Compound), 250X
- 9. DMSO for Compound Serial Dilution
- 10. Compound Dilution Buffer
- 11. Plate Sealers
Storage Conditions:
Store kit components under the conditions indicated on the labels. Discard any remaining reagents after 6 months.
Detection Methods:
Fluorescent
Antibody Category:
Neuroscience
Materials Required but Not Delivered:
1. Sterile, tissue culture-treated black/clear bottom microplates suitable for High-Content Imaging.
2. Cell-type for assay, e.g., HeLa (human cervical adenocarcinoma, ATCC #CCL-2) or A549 (human lung carcinoma, ATCC #CCL-185) or HepG2 (human hepatocellular carcinoma, ATCC #HB-8065).
3. Tissue culture instruments/supplies (including 37ºC incubator, growth media, flasks/plates, detachment buffer, etc.) for cell type of interest.
4. HCS imaging/analysis system, e.g., GE Healthcare IN Cell Analyzer 1000 with Investigator software. Imaging system must be equipped with beam-splitters and filters capable of reading emission spectra in the blue and red ranges.
2. Cell-type for assay, e.g., HeLa (human cervical adenocarcinoma, ATCC #CCL-2) or A549 (human lung carcinoma, ATCC #CCL-185) or HepG2 (human hepatocellular carcinoma, ATCC #HB-8065).
3. Tissue culture instruments/supplies (including 37ºC incubator, growth media, flasks/plates, detachment buffer, etc.) for cell type of interest.
4. HCS imaging/analysis system, e.g., GE Healthcare IN Cell Analyzer 1000 with Investigator software. Imaging system must be equipped with beam-splitters and filters capable of reading emission spectra in the blue and red ranges.