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Competition binding of MIP-1α to CCR1 Membrane Preparations. CCR1 Membrane Preparation (2.5 and 5 µg/well) or Wild-Type Chem-2 Membrane Preparation ...
ChemiSCREEN™ CCR1 Membrane Preparation
Description:
ChemiSCREEN™ CCR1 Membrane Preparation
Trade Name:
- ChemiScreen
- Chemicon (Millipore)
Qty/Pk:
1 mL
Product Overview:
Full-length human CCR1 cDNA
Background Information:
CCR1 is a GPCR that binds to a variety of CC ligands, including MIP-1a, RANTES, MCP-3, HCC-1, HCC-2, HCC-4, and MPIF-1 (Olson and Ley, 2002). Lymphocytes, macrophages, dendritic cells, and GM-CSF-activated neutrophils express CCR1 (Kaufmann et al., 2001; Cheng et al., 2001). Two selective, non-peptide small molecule antagonists of CCR1, BX-471 and CP-481,715, have been synthesized (Gladue et al., 2003; Liang et al., 2000). Pharmacological and genetic targeting of CCR1, either alone or in combination with cyclosporin A, reduces cardiac and renal allograft rejection (Gao et al., 2000; Horuk et al., 2001a; Horuk et al., 2001b), allergic encephalomyelitis (Liang et al., 2000), and renal fibrosis (Anders et al., 2002) in experimental models. Chemicon's CCR1 Membrane Preparations are ideal for screening molecules that disrupt interaction between CCR1 and its ligands. The CCR1 Membrane Preps bind to MIP-1a with a Kd of 1.8 nM, and yield greater than 10-fold signal to background ratio with a MIP-1a concentration of 0.1 nM.
Application Notes:
Radioligand binding assay.
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The ligands of this receptor include macrophage inflammatory protein 1 alpha (MIP-1 alpha), regulated on activation normal T expressed and secreted protein (RANTES), monocyte chemoattractant protein 3 (MCP-3), and myeloid progenitor inhibitory factor-1 (MPIF-1). Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. Knockout studies of the mouse homolog suggested the roles of this gene in host protection from inflammatory response, and susceptibility to virus and parasite. This gene and other chemokine receptor genes, including CCR2, CCRL2, CCR3, CCR5 and CCXCR1, are found to form a gene cluster on chromosome 3p.
UniProt Summary:
FUNCTION: SwissProt: P32246 # Receptor for a C-C type chemokine. Binds to MIP-1-alpha, MIP-1-delta, RANTES, and MCP-3 and, less efficiently, to MIP-1- beta or MCP-1 and subsequently transduces a signal by increasing the intracellular calcium ions level. Responsible for affecting stem cell proliferation.
SIZE: 355 amino acids; 41173 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely expressed in different hematopoietic cells.
SIMILARITY: SwissProt: P32246 ## Belongs to the G-protein coupled receptor 1 family.
SIZE: 355 amino acids; 41173 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely expressed in different hematopoietic cells.
SIMILARITY: SwissProt: P32246 ## Belongs to the G-protein coupled receptor 1 family.
Species:
Human
Brand Family:
Chemicon
Protein Target:
CCR1
Presentation:
Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
Each vial typically contains 500 units, where one unit is the amount of membrane prep that yields greater than 1000 cpm specific binding of 125I-labeled MIP-1α (0.1 nM input concentration).
Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
Each vial typically contains 500 units, where one unit is the amount of membrane prep that yields greater than 1000 cpm specific binding of 125I-labeled MIP-1α (0.1 nM input concentration).
Ligand Type:
peptide
Storage Conditions:
Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
Target Sub-Family:
Chemokine
GPCR Class:
A
Promotional Text:
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Gene Symbol:
- CCR1
- HM145
- CCR-1
- MIP1aR
- RANTES-R
- CKR-1
- CD191
- CMKBR1
- CC-CKR-1
- MIP-1alpha-R
- SCYAR1
- CMKR1
Protein or Enzyme Type:
GPCR Membrane Preparations
Product Name:
ChemiSCREEN™ CCR1 Membrane Preparation
Concentration:
0.5 mg/mL
Quality Assurance:
SPECIFICATIONS:
Bmax for [125I]MIP-1alpha binding: 3.3 pmol/mg protein
Kd for [125I] MIP-1alpha binding: ~ 1.8 nM HOST CELLS:
Bmax for [125I]MIP-1alpha binding: 3.3 pmol/mg protein
Kd for [125I] MIP-1alpha binding: ~ 1.8 nM HOST CELLS:
Cell Type:
Chem-2
Host Cells:
Chem-2
Format:
Membranes
UniProt Number:
Entrez Gene Number:
Cell Membrane Type:
GPCR