Content Loading...
Content Loading...
Product Images
Figure 1. Saturation binding for CCR7. 10 µg/well CCR7 Membrane Preparation was incubated with increasing amount of [125I]- MIP-3β in the...
Figure 2. Competition binding for CCR7. CCR7 Membrane Preparation (10 µg/well) was incubated with 0.1 nM [125I]-MIP-3β and increasing co...
ChemiSCREEN™ CCR7 Membrane Preparation, recombinant
Description:
ChemiSCREEN™ CCR7 Membrane Preparation, recombinant
Trade Name:
- ChemiScreen
- Chemicon (Millipore)
Qty/Pk:
200 assays
Product Overview:
Full-length human CCR7 cDNA
Background Information:
CCR7 is a GPCR that binds to CC chemokine ligands MIP-3beta (ELC/CCL19) and 6Ckine (CCL21)1. These ligands are expressed in the secondary lymphoid organs, and binding to CCR7 expressed in naïve T cells, B cells and dendritic cells directs migration of these cells to sites of antigen presentation2. Inhibition of CCR7/ligand interactions inhibits contact sensitivity, delayed type hypersensitivity, and graft vs host disease in experimental models2,3. In addition, CCR7 expression by breast cancer, melanoma and other malignant cells are associated with lymph node metastasis4,5. Chemicon's CCR7 Membrane Preparations are ideal for screening for inhibitors of CCR7/ligand interactions. The CCR7 membrane preparations bind to MIP-3beta with a Kd of approximately 0.2 nM. At a [125I]-MIP-3beta concentration of 0.1 nM, a signal to background ratio of greater than 8-fold is typically obtained.
Application Notes:
Radioligand binding assay and GTPγS binding
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor.
UniProt Summary:
FUNCTION: SwissProt: P32248 # Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
SIZE: 378 amino acids; 42874 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
SIMILARITY: SwissProt: P32248 ## Belongs to the G-protein coupled receptor 1 family.
SIZE: 378 amino acids; 42874 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
SIMILARITY: SwissProt: P32248 ## Belongs to the G-protein coupled receptor 1 family.
Species:
Human
Brand Family:
Chemicon
Protein Target:
CCR7
Presentation:
Membranes were adjusted to 0.5 mg protein/ml in 50 mM Tris pH 7.4, 10% glycerol and 1% BSA, rapidly frozen, and stored at -80°C, sufficient for 200 microassays per vial.
Ligand Type:
peptide
Storage Conditions:
Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
Target Sub-Family:
Chemokine
GPCR Class:
A
Promotional Text:
For special offers Click here http://www.millipore.com/drugdiscovery/dd3/gpcrtargetsolutions
Gene Symbol:
- CCR7
- CMKBR7
- EBI1
- CD197
- CDw197
- CCR-7
- BLR2
- EVI1
- CC-CKR-7
Protein or Enzyme Type:
GPCR Membrane Preparations
Product Name:
ChemiSCREEN™ CCR7 Membrane Preparation, recombinant
Quality Assurance:
SPECIFICATIONS: 1 unit = 10 µg membrane preparation
Bmax: 3.3 pmol/mg
Kd: 0.2 nM
Table 1. Signal:background and specific binding values obtained in a competition binding assay with CCR7 membrane preparation.
Bmax: 3.3 pmol/mg
Kd: 0.2 nM
| 10 µg/well | |
|---|---|
| Signal:Background | 10.2 |
| Specific Binding (cpm) | 8362 |
Cell Type:
Chem-2
Incubation Conditions:
Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I] MIP-3β (Perkin Elmer #NEX370 )
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 7-fold signal:background with [125I]-labeled MIP-3β at 0.1 nM
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I] MIP-3β (Perkin Elmer #NEX370 )
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 7-fold signal:background with [125I]-labeled MIP-3β at 0.1 nM
Host Cells:
Chem-2
Format:
Membranes
UniProt Number:
Entrez Gene Number:
Cell Membrane Type:
GPCR