ChemiSCREEN™ CX3CR1 Calcium-Optimized FLIPR Cell Line

ChemiSCREEN™ CX3CR1 Calcium-Optimized FLIPR Cell Line

• ChemiScreen
• Chemicon (Millipore)

2 vials

Full-length Human CX3CR1

CX₃CR1 is a GPCR expressed on natural killer cells, cytotoxic T lymphocytes, and macrophages. The sole ligand for CX₃CR1, fractalkine, is an unusual chemokine that is expressed as a transmembrane molecule with a CX3C domain and a mucin domain (Imai et al., 1997). Fractalkine is highly expressed on endothelial cells activated by TNFα and other proinflammatory cytokines, and fractalkine/CX₃CR1 interactions mediate recruitment of macrophages into the atherosclerotic plaque (Lesnick et al., 2003; McDermott et al., 2003). In addition, fractalkine and CX₃CR1 have been implicated in the pathogenesis of glomerulonephritis, HIV infection and rheumatoid arthritis (Ito et al., 2002; Faure et al., 2003; Nanki et al., 2002). Chemicon's cloned human CX₃CR1 -expressing cell line is made in the Chem-4 host, which supports high levels of recombinant CX₃CR1 expression on the cell surface and contains high levels of promiscuous G proteins to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CX₃CR1 and its ligands.

Human

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

FUNCTION: SwissProt: P49238 # Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV coreceptors than isoform 1.
SIZE: 355 amino acids; 40396 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in lymphoid and neural tissues.
SIMILARITY: SwissProt: P49238 ## Belongs to the G-protein coupled receptor 1 family.

Human

Chemicon

CX3CR1

Cells are frozen at 2 x 10⁶ cells/mL in DMEM/20% fetal bovine serum/100U/mL each penicillin and streptomycin/10% DMSO. Cell line tests negative for mycoplasma.

peptide

Place cells in liquid nitrogen immediately upon receipt. Maintain frozen in liquid nitrogen for up to 5 years. Thaw cells rapidly by removing from liquid nitrogen and immediately immersing in a 37°C water bath. Immediately after ice has thawed, sterilize the exterior of the vial with 70% ethanol. Transfer contents of the vial to a T75 flask containing growth media, and replace media after 8-24 h. Cells are typically passaged 1:10 with 0.05% Trypsin/0.2 g/L EDTA every 3-4 days, and should be passaged at least once after thawing prior to use in calcium flux assays.

Chemokine

A

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• CX3CR1
• CMKDR1
• GPR13
• CMK-BRL-1
• V28
• GPRV28
• CMKBRL1
• CCRL1
• CMKBLR1

GPCRs

ChemiSCREEN™ CX3CR1 Calcium-Optimized FLIPR Cell Line

V28

DMEM containing 4.5 g/L glucose/10% heat inactivated fetal bovine serum/1x nonessential amino acids/10 mM HEPES/ 0.25 mg/ml Geneticin (G418)/ 0.5 mg/ml

2 x 10⁶ cells/mL

EC50 for calcium mobilization by Fractalkine: 72.8 nM

Signal/noise ratio: 586

Chem-4

GPCR Cell Lines

Chem-4

Cell Line

P49238

2x10⁶ cells/vial

NM_001337.3