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Figure 1. Competition binding to C3aR. 5 micrograms/well C3aR Membrane Preparation was incubated with 0.04 nM [125I]-C3a and increasing concentratio...
ChemiSCREEN™ C3aR Membrane Preparation
Description:
ChemiSCREEN™ C3aR Membrane Preparation
Trade Name:
- ChemiScreen
- Chemicon (Millipore)
Qty/Pk:
1 mL
Product Overview:
Full-length human C3aR cDNA
Background Information:
C3a, along with C4a and C5a, is a 77 amino acid anaphylotoxin generated by proteolytic cleavage during activation of the complement pathway (Ember, 1997). The anaphylotoxins strongly promote inflammation by recruiting leukocytes, particularly basophils, eosinophils, neutrophils and monocyte (Martin, 1997). The proinflammatory properties of C3a are mediated by interaction between the peptide and a 7-TM G-protein coupled receptor, C3aR (Crass, 1996). Genetic and pharmacological inhibition of C3a/C3aR interaction indicates an important role for C3aR in allergic asthsma Humbles, 2000; Drouin, 2002). C3a/C3aR also enhances the effect of SDF-1 in promoting retention of haematopoietic stem/progenitor cells within the bone marrow (Rataiczak, 2004). Chemicon's C3aR membrane preps are an ideal tool for screening for compounds that disrupt binding of C3a to C3aR. The membranes have been shown to bind to 125I-labeled C3a with a Kd of 0.06 nM.
Application Notes:
Radioligand binding assay
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UniProt Summary:
FUNCTION: SwissProt: Q16581 # Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
SIZE: 482 amino acids; 53864 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues.
PTM: Among the sulfation sites Tyr-174 is essential for binding of C3a anaphylatoxin.
SIMILARITY: SwissProt: Q16581 ## Belongs to the G-protein coupled receptor 1 family.
SIZE: 482 amino acids; 53864 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues.
PTM: Among the sulfation sites Tyr-174 is essential for binding of C3a anaphylatoxin.
SIMILARITY: SwissProt: Q16581 ## Belongs to the G-protein coupled receptor 1 family.
Species:
Human
Brand Family:
Chemicon
Protein Target:
C3aR
Presentation:
Membranes were adjusted to 0.5mg protein/mL in 50 mM Tris-HCL, pH 7.4, 10% glycerol and 1% BSA, rapidly frozen, and stored at -80°C. One vial contains 500 units, where a unit is the amount of membrane that will yield greater than 1000 cpm specific binding of 125I-labeled C3a (0.04 nM input concentration).
Ligand Type:
peptide
Storage Conditions:
Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
Target Sub-Family:
Anaphylotoxin
GPCR Class:
A
Promotional Text:
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Gene Symbol:
- C3AR1
- C3a-R
- HNFAG09
- C3AR
- C3R1
- AZ3B
Protein or Enzyme Type:
GPCR Membrane Preparations
Product Name:
ChemiSCREEN™ C3aR Membrane Preparation
Concentration:
0.5mg/ml
Quality Assurance:
Bmax for [125I]C3a binding: 1.9 pmol/mg protein
Kd for [125I] C3a binding: ~ 0.06 nM
Kd for [125I] C3a binding: ~ 0.06 nM
Cell Type:
Chem-2
Host Cells:
Chem-2
Format:
Membranes
UniProt Number:
Entrez Gene Number:
Cell Membrane Type:
GPCR