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Figure 1. Saturation binding for C5aR. 10 microgram/well of C5aR Membrane Preparation was incubated with [125I]-C5a in the absence (TB=total binding...
Figure 2. Competition binding for C5aR. 5 micrograms/well C5aR Membrane Preparation was incubated with 0.1 nM 125I-labeled C5a and increasing concen...
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ChemiSCREEN™ C5aR Membrane Preparation, recombinant
Description:
ChemiSCREEN™ C5aR Membrane Preparation, recombinant
Trade Name:
- ChemiScreen
- Chemicon (Millipore)
Qty/Pk:
200 assays
Product Overview:
Full-length human C5aR cDNA
Background Information:
C5a is a proinflammatory peptide generated through activation of the complement system, and is more potent than the other anaphylatoxins, C4a and C3a, in activating peripheral blood leukocytes (Gerard & Gerard, 1994). It can actively participate in the regulation of local cytokine network by stimulating production of proinflammatory cytokines (Buchner et al., 1995; Hsu, et al., 1999). The proinflammatory effects of C5a are mediated through binding to a specific 7-TM chemoattractant receptor, C5aR (CD88). Binding of C5a to phagocyte C5aR induces chemotaxis, production of superoxide anions, and release of degradative enzymes. Pharmacologic or genetic disruption of C5a/C5aR interaction reduces sepsis (Riedemann et al., 2003), immune complex-induced lung disease (Shushakova et al., 2002), and Arthrogen-induced arthritis (Grant et al., 2002) in experimental models. Chemicon's cloned human C5a receptor membrane preparations are ideal tools for screening for antagonists of C5a/C5aR interactions. The C5aR Membrane Preps bind specifically to 125I-labeled ligand C5a, with Kd of 0.59 nM. With 5 μg membrane per well and 0.1 nM 125I-labeled C5a, more than 15-fold signal:background was obtained.
Application Notes:
Radioligand binding assay (See images & graphs link)
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UniProt Summary:
FUNCTION: SwissProt: P21730 # Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
SIZE: 350 amino acids; 39321 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
PTM: Sulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.
SIMILARITY: SwissProt: P21730 ## Belongs to the G-protein coupled receptor 1 family.
SIZE: 350 amino acids; 39321 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
PTM: Sulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.
SIMILARITY: SwissProt: P21730 ## Belongs to the G-protein coupled receptor 1 family.
Species:
Human
Brand Family:
Chemicon
Protein Target:
C5aR
Presentation:
Membranes were adjusted to 0.5 mg protein/ml in 50 mM Tris pH 7.4, 10% glycerol and 1% BSA, rapidly frozen, and stored at -80°C. One vial contains 1000 units, where a unit is the amount of membrane that will yield greater than 1000 cpm specific binding of I-125 labeled C5a (0.1 nM input concentration).
Ligand Type:
peptide
Storage Conditions:
Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
Target Sub-Family:
Anaphylotoxin
GPCR Class:
A
Promotional Text:
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Gene Symbol:
- C5AR1
- CD88
- C5AR
- C5A
- C5R1
- C5aR
- C5a-R
Protein or Enzyme Type:
GPCR Membrane Preparations
Product Name:
ChemiSCREEN™ C5aR Membrane Preparation, recombinant
Concentration:
0.5 mg/mL
Quality Assurance:
SPECIFICATIONS: Bmax for [125I]C5a binding: 9.7 pmol/mg protein
Kd for [125I] C5a binding: ~ 0.59 nM
Kd for [125I] C5a binding: ~ 0.59 nM
Cell Type:
Chem-1
Host Cells:
Chem-1
Format:
Membranes
UniProt Number:
Entrez Gene Number:
Cell Membrane Type:
GPCR